Lin C W, Chapman C M, DeLellis R A, Kirley S
J Histochem Cytochem. 1978 Nov;26(11):1021-5. doi: 10.1177/26.11.102687.
An immunofluorescent procedure for the localization of histaminase in human tissue sections has been developed by using a specific antiserum against human placental histaminase. For localization of this enzyme in placental sections, fixation in equal volumes mixture of absolute ethanol and acetone provided the optimum visualization of this enzyme in both frozen sections and paraffin-embedded sections. The immunofluorescent staining of this enzyme in placenta is found to be localized in areas within the maternal decidua, both within the cytoplasm of the decidual cells and in tissue space between the cells. The chorionic villi are completely void of the immunofluorescent stain. Variations in patterns of histaminase localization have been found between term and premature placentas, with the former showing a predominantly intercellular localization and the latter a predominantly intracellular localization. The intercellular localization of this enzyme in the decidua may represent a nonspecific diffusion of the enzyme associated with delivery of the placenta or may reflex a specific functional role of the enzyme in the intercellular space during pregnancy.
通过使用针对人胎盘组胺酶的特异性抗血清，已开发出一种用于在人体组织切片中定位组胺酶的免疫荧光方法。为了在胎盘切片中定位这种酶，用无水乙醇和丙酮等体积混合固定，能使该酶在冰冻切片和石蜡包埋切片中都获得最佳的可视化效果。发现该酶在胎盘中的免疫荧光染色定位于母体蜕膜内的区域，在蜕膜细胞的细胞质内和细胞间的组织间隙中。绒毛膜绒毛完全没有免疫荧光染色。已发现足月胎盘和早产胎盘之间组胺酶定位模式存在差异，前者主要表现为细胞间定位，后者主要表现为细胞内定位。该酶在蜕膜中的细胞间定位可能代表与胎盘娩出相关的酶的非特异性扩散，也可能反映该酶在孕期细胞间空间中的特定功能作用。
