Weisburger W R, Mendelsohn G, Eggleston J C, Baylin S B
Lab Invest. 1978 Jun;38(6):703-6.
An immunohistochemical technique has been developed for the localization of histaminase (diamine oxidase) in human placenta. The procedure utilizes antibody to highly purified human placental enzyme prepared in rabbits. Histaminase is shown to be present in the cytoplasm of the decidual cells of the maternal placenta; the staining is specific and can be removed by preabsorption of the antisera with purified antigen. The present demonstration of histaminase in the maternal portion of placenta confirms previous biochemical data suggesting that the enzyme is not a fetal product in this tissue. This study also provides further evidence that the presence of histaminase in some human tumors and in normal tissues like kidney and intestine is the result of the expression of a mature and not a fetal genome.
已开发出一种免疫组织化学技术，用于在人胎盘中定位组胺酶（二胺氧化酶）。该程序利用针对在兔体内制备的高度纯化的人胎盘酶的抗体。结果表明，组胺酶存在于母体胎盘蜕膜细胞的细胞质中；染色具有特异性，并且可以通过用纯化抗原预先吸收抗血清来去除。目前在胎盘母体部分中组胺酶的证明证实了先前的生化数据，表明该酶在该组织中不是胎儿产物。这项研究还提供了进一步的证据，表明组胺酶在某些人类肿瘤以及肾脏和肠道等正常组织中的存在是成熟而非胎儿基因组表达的结果。
