Bulatov A A, Demina N A, Pankov Iu A
Biokhimiia. 1976 Jul;41(6):1146-9.
A simple method of isolation of highly purified prolactin from acetonated preparations of anterior hypophysial lobes is described. The new method permits to obtain higher (about 10-fold) yields of the hormone, as compared to those obtained using previously described methods. Prolactin was extracted by acid aqueous acetone and was subsequently purified of extract by fractionation with acetone and NaCl and by isoelectric precipitation. The final stage of the hormone purification involved gel-filtration through Sephadex G-200; prolactin yield was 400 microgram per 1 hypophysis. The lactogenic activity of the hormone is 14 MU/mg; the sequence of N-terminal amino acid residues of prolactin is as follows: NH2-Leu-Pro-Ile-x-Pro-Leu(?)-Gly-Ala-.
本文描述了一种从垂体前叶丙酮化制剂中分离高纯度催乳素的简单方法。与使用先前描述的方法相比，新方法能获得更高（约10倍）的激素产量。催乳素用酸性丙酮水溶液提取，随后通过丙酮和氯化钠分级分离以及等电沉淀从提取物中纯化。激素纯化的最后阶段是通过Sephadex G - 200进行凝胶过滤；每1个垂体的催乳素产量为400微克。该激素的催乳活性为14 MU/mg；催乳素N端氨基酸残基序列如下：NH2 - Leu - Pro - Ile - x - Pro - Leu(?) - Gly - Ala - 。
