大西洋鲑鱼催乳素的纯化与特性分析

Purification and characterization of Atlantic salmon prolactin.

作者信息

Andersen O, Skibeli V, Gautvik K M

机构信息

Institute of Medical Biochemistry, University of Oslo, Norway.

出版信息

Gen Comp Endocrinol. 1989 Mar;73(3):354-60. doi: 10.1016/0016-6480(89)90191-3.

DOI:10.1016/0016-6480(89)90191-3

PMID:2925076

Abstract

Prolactin was isolated from the Atlantic salmon (Salmo salar) pituitary gland by extraction with acid acetone, gel filtration, ion exchange-chromatography, and reversed-phase high-performance liquid chromatography. The yield was 0.6 mg/g wet tissue. The hormone had a molecular weight of 23.5 kDa as determined by SDS-polyacrylamide gel electrophoresis. Isoelectric focusing gave an isoelectric point of 9.2. The N-terminal sequence and the amino acid composition indicated extensive homology between Atlantic and Pacific salmon prolactin. Antiserum against Atlantic salmon prolactin cross-reacted with chum salmon prolactin, but not with human, rat, or sheep prolactin.

摘要

通过用酸性丙酮萃取、凝胶过滤、离子交换色谱和反相高效液相色谱法，从大西洋鲑鱼（Salmo salar）的垂体中分离出催乳素。产量为0.6毫克/克湿组织。通过SDS-聚丙烯酰胺凝胶电泳测定，该激素的分子量为23.5 kDa。等电聚焦给出的等电点为9.2。N端序列和氨基酸组成表明，大西洋鲑鱼和太平洋鲑鱼的催乳素之间存在广泛的同源性。抗大西洋鲑鱼催乳素的抗血清与大麻哈鱼催乳素发生交叉反应，但与人类、大鼠或绵羊的催乳素不发生交叉反应。