Butnev V Iu, Pankov Iu A
Biokhimiia. 1984 Nov;49(11):1828-39.
Different forms of prolactin obtained from porcine hypophysis and differing in terms of molecular weight, electrophoretic mobility and biological activity were studied by polyacrylamide gel electrophoresis. A glycosylated form of prolactin with Mr 25 000 and possessing an increased biological activity towards pigeon crop was revealed. It was found that the carbohydrate component of this prolactin form is attached to asparagine at position 31; no differences were revealed between the amino acid composition of the major and glycosylated forms of the hormone. In the hypophysis, the glycosylated form content makes up to 30-40% of the total prolactin monomer content. A disulfide dimeric form of prolactin with Mr of about 50 000 was isolated and characterized.
通过聚丙烯酰胺凝胶电泳研究了从猪垂体获得的不同形式的催乳素，这些催乳素在分子量、电泳迁移率和生物活性方面存在差异。发现了一种分子量为25000的糖基化催乳素形式，其对鸽嗉囊的生物活性增强。研究发现，这种催乳素形式的碳水化合物成分连接在第31位的天冬酰胺上；该激素的主要形式和糖基化形式在氨基酸组成上没有差异。在垂体中，糖基化形式的含量占催乳素单体总含量的30%-40%。分离并鉴定了一种分子量约为50000的二硫键二聚体形式的催乳素。
