Woode G N, Bridger J C
J Med Microbiol. 1978 Nov;11(4):441-52. doi: 10.1099/00222615-11-4-441.
Small round viruses (SRV) were isolated from the faeces of diarrhoeic calves from three farms. All three SRV preparations caused diarrhoea experimentally in gnotobiotic calves. Each preparation contained viral particles of two morphological types, "astrovirus-like" and "calicivirus-like", and from one preparation the two particle types were separated from each other. The calicivirus-like agent ("Newbury agent") was 33 nm in diameter, and caused diarrhoea in gnotobiotic calves with villous atrophy and D-xylose malabsorption. This virus did not infect cell cultures. The astrovirus-like agent did not cause diarrhoea in two gnotobiotic calves; however, it infected cell cultures (primary calf kidney) and the infected cells immunofluoresced with convalescent gnotobiotic-calf antiserum. The astrovirus-like agents in the three preparations were antigenically related. Experiments in calves showed that there was a degree of cross-protection between the three SRV preparations, as judged by the presence or absence of diarrhoea, but that at least three unrelated pathogens were present.
从小牛腹泻的三个农场的腹泻小牛粪便中分离出小圆病毒（SRV）。所有三种SRV制剂在无菌小牛中均可引发实验性腹泻。每种制剂都含有两种形态类型的病毒颗粒，“类星状病毒”和“类杯状病毒”，并且从一种制剂中分离出了这两种颗粒类型。类杯状病毒制剂（“纽伯里制剂”）直径为33纳米，可导致无菌小牛出现腹泻，并伴有绒毛萎缩和D-木糖吸收不良。这种病毒不会感染细胞培养物。类星状病毒制剂在两只无菌小牛中未引发腹泻；然而，它感染了细胞培养物（原代小牛肾），并且感染的细胞在用恢复期无菌小牛抗血清进行免疫荧光检测时呈阳性。三种制剂中的类星状病毒制剂在抗原上具有相关性。在小牛身上进行的实验表明，根据腹泻的有无判断，三种SRV制剂之间存在一定程度的交叉保护，但至少存在三种不相关的病原体。
