Uzbekov M G, Ivanova T M
Biull Eksp Biol Med. 1976 Oct;82(10):1209-11.
Composition of membrane proteins of the visual system structures of normal and dark-reared rabbits (for 2 1/2 months from birth) was studied by using polyacrylamide gel electrophoresis. Solubilization of membrane proteins was carried out using a 1% solution of Triton X-100 and a 0.1% solution of sodium dodecylsulfate, in succession. One fraction representing the fraction of high-molecular proteins was absent in membrane proteins of the visual analyser in the dark-reared animals; there was also a change in the percentage content of proteins in different fractions in comparison with the normal. The data obtained led to the conclusion that visual deprivation caused marked quantitative and qualitative reconstruction in the composition of membrane proteins of the brain with some specific characteristics for the formation of the visual analyser.
通过聚丙烯酰胺凝胶电泳研究了正常饲养和出生后2个半月黑暗饲养的家兔视觉系统结构的膜蛋白组成。依次使用1%的 Triton X-100溶液和0.1%的十二烷基硫酸钠溶液对膜蛋白进行增溶。在黑暗饲养动物的视觉分析器的膜蛋白中，代表高分子蛋白部分的一个组分缺失；与正常情况相比，不同组分中蛋白质的百分比含量也发生了变化。所获得的数据得出结论，视觉剥夺导致大脑膜蛋白组成发生显著的定量和定性重构，具有视觉分析器形成的一些特定特征。
