丁香中乙酰胆碱酯酶抑制剂的生化研究。

Biochemical studies of acetylcholine esterase inhibitor present in Eugenia caryophyllus.

作者信息

Akinrimisi E O, Akinwande A I

出版信息

West Afr J Pharmacol Drug Res. 1976 Dec;3(2):141-8.

Abstract

Water, saline and ethanol extracts of Eugenia caryophyllus inhibited brain acetylcholinesterase (AchE) activity. Inhibitory action of the saline extract was greater than that of other extracts. The most active fraction of the saline extract obtained by chromatography through Sephadex G25 and DEAE cellulose columns and elution with tris and phosphate buffers (pH. 7.1) exhibited reactions characteristic of phenols, amino compounds and uronic acid. It is, therefore, suggested that the active inhibitor is a complex glycoside containing phenol and uronic acid part structure.

摘要

丁香的水提取物、盐水提取物和乙醇提取物均可抑制脑乙酰胆碱酯酶（AchE）的活性。盐水提取物的抑制作用强于其他提取物。通过Sephadex G25和DEAE纤维素柱色谱法，并用tris和磷酸盐缓冲液（pH 7.1）洗脱得到的盐水提取物中最具活性的部分表现出酚类、氨基化合物和糖醛酸的反应特征。因此，推测活性抑制剂是一种含有酚和糖醛酸部分结构的复合糖苷。