Olgiati D D, Pogo B G, Dales S
J Cell Biol. 1976 Mar;68(3):557-66. doi: 10.1083/jcb.68.3.557.
Rapidly labeled, i.e., nascent, DNA from HeLa cells was separated from the bulk DNA by ultracentrifugation. Further characterization of the rapidly labeled component revealed that its sedimentation coefficient is in the range of 4S and that it exists in a single- and double-stranded conformation. Moreover, analysis by nitrocellulose chromatography and CsSO4 density sedimentation of the nascent DNA labeled with 3H-uridine revealed that it is covalently linked to short chains of RNA, indicating that in HeLa cells RNA primer is involved in DNA replication.
通过超速离心将来自HeLa细胞的快速标记(即新生)DNA与大量DNA分离。对快速标记成分的进一步表征显示,其沉降系数在4S范围内,并且以单链和双链构象存在。此外,通过硝酸纤维素色谱法和对用³H-尿苷标记的新生DNA进行CsSO₄密度沉降分析表明,它与短链RNA共价连接,这表明在HeLa细胞中RNA引物参与DNA复制。