Kobayakawa T, Shinbo S, Ishiyama H, Masuda K
Jpn J Med Sci Biol. 1976 Dec;29(6):351-7. doi: 10.7883/yoken1952.29.351.
Parasiticidal activity of normal peritoneal exudate cells on microfilariae of Dirofilaria immitis in diffusion chambers implanted into normal guinea pigs was evoked by intraperitoneal passive transfer of anti-D. immitis serum. The immune serum was fractionated into supernatant and sediment by ammonium sulfate precipitation. The parasiticidal effect was reproduced with the supernatant and, to a less extent, with the sediment. Anti-D. immitis serum from the animals sensitized 5 days before was also effective in this respect. From these results it can be concluded that the factor responsible for the phenomenon is some other factor(s) than the antibody. In addition, the in vitro cytotoxicity test demonstrated an enhanced Mf-killing activity of sensitized peritoneal exudate cells by adding with anti-D. immitis serum.
通过腹腔被动转移抗犬恶丝虫血清，可诱发正常豚鼠腹腔内植入扩散小室中的正常腹腔渗出细胞对犬恶丝虫微丝蚴的杀寄生虫活性。通过硫酸铵沉淀将免疫血清分为上清液和沉淀物。上清液可重现杀寄生虫作用，沉淀物的作用程度较小。在这方面，来自5天前致敏动物的抗犬恶丝虫血清也有效。从这些结果可以得出结论，导致该现象的因素是除抗体以外的其他某种因素。此外，体外细胞毒性试验表明，添加抗犬恶丝虫血清可增强致敏腹腔渗出细胞的杀微丝蚴活性。
