Rzepczyk C M, Bishop C J, Cheung K, Atwell R, Ferrante A
Aust J Exp Biol Med Sci. 1986 Feb;64 ( Pt 1):43-51. doi: 10.1038/icb.1986.5.
It has been shown that the interaction of Dirofilaria immitis microfilariae (Mf) opsonized with sera from infected but amicrofilaraemic dogs (occult dogs) stimulated the respiratory burst and degranulation of neutrophils as measured by chemiluminescence and iodination. Sera from normal and microfilaraemic dogs gave either low level or non-significant reactions. Since the sera required were also those required for neutrophil-mediated cytotoxicity to D. immitis Mf in vitro, the results suggested that the products of oxygen reduction as well as the myeloperoxidase system could be involved in the killing of Mf by neutrophils. However, whether these pathways have a major role to play in neutrophil-mediated cytotoxicity to Mf is uncertain, as various chemical and enzymatic inhibitors of the products of the respiratory burst were unable to prevent or reduce cytotoxicity. Azide, which is a known inhibitor of the iodination reaction, also failed to reduce cytotoxicity.
研究表明，用感染但无微丝蚴血症犬（隐匿犬）的血清调理的犬恶丝虫微丝蚴（Mf）的相互作用，通过化学发光和碘化法测定，刺激了中性粒细胞的呼吸爆发和脱颗粒。正常犬和微丝蚴血症犬的血清产生的反应水平较低或无显著反应。由于所需的血清也是体外中性粒细胞介导的对犬恶丝虫Mf细胞毒性所需的血清，结果表明，氧还原产物以及髓过氧化物酶系统可能参与中性粒细胞对Mf的杀伤。然而，这些途径是否在中性粒细胞介导的对Mf的细胞毒性中起主要作用尚不确定，因为呼吸爆发产物的各种化学和酶抑制剂无法预防或降低细胞毒性。叠氮化物是一种已知的碘化反应抑制剂，也未能降低细胞毒性。
