Garrido J
Arch Biol Med Exp. 1976 Dec;10(1-3):100-4.
Labeling of lectin receptors during the cell cycle. (Localizabión de receptores para lectinas durante el ciclo celular). Arch. Biol. Med. Exper. 10: 100-104, 1976. The topographic distribution of specific cell surface receptors for concanavalin A and wheat germ agglutinin was studied by ultrastructural labeling in the course of the cell cycle. C12TSV5 cells were synchronized by double thymidine block or mechanical selection (shakeoff). They were labeled by means of lectin-peroxidase techniques while in G1 S, G2 and M phases of the cycle. The results obtained were similar for both lectins employed. Interphase cells (G1 S, G2) present a stlihtly discontinous labeling pattern that is similar to the one observed on unsynchronized cells of the same line. Cells in mitosis, on the contrary, present a highly discontinous distribution of reaction product. This pattern disappears after the cells enters G1 and is not present on mitotic cells fixed in aldehyde prior to labeling.
细胞周期中凝集素受体的标记。（细胞周期中凝集素受体的定位）。《实验生物学与医学档案》10: 100 - 104, 1976年。通过超微结构标记研究了在细胞周期过程中伴刀豆球蛋白A和麦胚凝集素的特异性细胞表面受体的拓扑分布。C12TSV5细胞通过双胸腺嘧啶核苷阻断或机械选择（摇落法）进行同步化。在细胞周期的G1、S、G2和M期，它们通过凝集素-过氧化物酶技术进行标记。对于所使用的两种凝集素，获得的结果相似。间期细胞（G1、S、G2）呈现出一种轻微不连续的标记模式，这与在同一细胞系的非同步化细胞上观察到的模式相似。相反，有丝分裂期的细胞呈现出反应产物的高度不连续分布。这种模式在细胞进入G1期后消失，并且在标记前用醛固定的有丝分裂细胞上不存在。
