Roth J, Wagner M
Exp Pathol (Jena). 1977;14(6):311-20. doi: 10.1016/s0014-4908(77)80050-1.
The redistribution and internalisation of two different lectin-binding sites on the same cell was investigated electron microscopically on unfixed rat liver cell cultures. For these purposes an electron microscopic double labeling technique was used for visualisation of the anti-AHel-binding sites by the gold-labeled lectin and for the demonstration of the Concanavalin A-binding sites by the Concanavalin A-peroxidase technique. From the experiments it was evident that the ligand-induced redistribution of the antiAHel-binding sites effects a rearrangement of the Concanavalin A-binding sites. Both markers were found in a clustered distribution on the cell surface. Following the redistribution an internalisation of both lectin-binding sites could be observed on central and peripheral cell parts. In the lateral cell parts an accumulation of the both markers occurred on the plasma membrane.
在未固定的大鼠肝细胞培养物上，利用电子显微镜研究了同一细胞上两个不同凝集素结合位点的重新分布和内化。为此，采用电子显微镜双标记技术，通过金标记凝集素可视化抗AHel结合位点，并通过伴刀豆球蛋白A-过氧化物酶技术展示伴刀豆球蛋白A结合位点。从实验中可以明显看出，抗AHel结合位点的配体诱导重新分布会影响伴刀豆球蛋白A结合位点的重排。两种标记物在细胞表面呈聚集分布。重新分布后，在细胞中央和周边部分均可观察到两种凝集素结合位点的内化。在细胞侧面部分，两种标记物在质膜上发生聚集。
