Kato K, Iwata S, Suginaka H, Namba K, Kotani S
Biken J. 1976 Dec;19(4):139-50.
The chemical structure of the cell wall peptidoglycan of Vibrio parahaemolyticus A55 was studied. Estimation of cross linkages between peptide subunits in the peptidoglycan by dinitrophenylation showed that about 30% of the total 2,6-diaminopimelic acid (A2pm) residues were involved in cross linkages. The presence of interpeptide bridges was also demonstrated by isolating bisdisaccharide peptide subunit dimers from Chalaropsis muramidase digests of the cell wall peptidoglycan by gel filtration followed by ion-exchange column chromatography, although most of the building blocks obtained were uncross-linked disaccharide peptide monomers. The chain length of a glycan moiety of the peptidoglycan obtained by treatment with the L-11 enzyme and gel filtration of the digest was also studied. The chain length varied from 7 to 44, but 30% of the glycan fragments had muramic acid at the reducing end and a chain length of 28 to 44. In conformity with the above structural study it was demonstrated that a particulate enzyme fraction obtained by differential centrifugation of a sonicated preparation of V. parahaemolyticus catalyzed a penicillin-sensitive transpeptidation reaction, using UDP-MurNAc-14C-pentapeptide and UDP-GlcNAc as substrates.
对副溶血性弧菌A55细胞壁肽聚糖的化学结构进行了研究。通过二硝基苯化法对肽聚糖中肽亚基之间的交联进行估计，结果表明，在总的2,6-二氨基庚二酸（A2pm）残基中，约30%参与了交联。通过凝胶过滤，然后进行离子交换柱色谱，从细胞壁肽聚糖的Chalaropsis溶菌酶消化物中分离出双二糖肽亚基二聚体，也证明了肽间桥的存在，尽管获得的大多数结构单元是未交联的二糖肽单体。还研究了用L-11酶处理并对消化物进行凝胶过滤后得到的肽聚糖聚糖部分的链长。链长从7到44不等，但30%的聚糖片段在还原端含有胞壁酸，链长为28到44。与上述结构研究一致，结果表明，通过对副溶血性弧菌超声破碎制剂进行差速离心得到的颗粒酶部分，以UDP-MurNAc-14C-五肽和UDP-GlcNAc为底物，催化青霉素敏感的转肽反应。
