Severin A I, Kokeguchi S, Kato K
Department of Microbiology, Okayama University Dental School, Japan.
Arch Microbiol. 1989;151(4):348-52. doi: 10.1007/BF00406563.
The mechanism of lysis of Eubacterium alactolyticum cell walls by Streptomyces albus G enzyme was studied. The analysis of the peptide terminal groups and peptide subunits isolated from the cell wall digest, released during solubilization of the cell walls, revealed that lytic action of S. albus G enzyme was mainly due to D-alanyl-A2pm endopeptidase, N-acetylmuramyl-L-alanine amidase, N-acetylmuramidase and N-acetylglucosaminidase. E. alactolyticum cell wall peptidoglycan is composed mainly of glucosamine, muramic acid, D-glutamic acid, L- and D-alanine, meso-diaminopimelic acid and glycine. The peptide subunit consists of L-alanyl-D-glutamyl-meso-A2pm-D-alanine. D-Alanine is connected directly with the amino group of the meso-A2pm residue of another peptide subunit. All of the L-amino groups of meso-diaminopimelic acid are involved in cross-linking. The possible structure of the peptide moiety of E. alactolyticum cell wall peptidoglycan is presented.
研究了白色链霉菌G酶对解乳糖优杆菌细胞壁的裂解机制。对细胞壁消化产物（细胞壁溶解过程中释放）中分离出的肽末端基团和肽亚基进行分析，结果表明白色链霉菌G酶的裂解作用主要归因于D-丙氨酰-A2pm内肽酶、N-乙酰胞壁酰-L-丙氨酸酰胺酶、N-乙酰胞壁酶和N-乙酰葡糖胺酶。解乳糖优杆菌细胞壁肽聚糖主要由葡糖胺、胞壁酸、D-谷氨酸、L-和D-丙氨酸、内消旋二氨基庚二酸和甘氨酸组成。肽亚基由L-丙氨酰-D-谷氨酰-内消旋-A2pm-D-丙氨酸组成。D-丙氨酸直接与另一个肽亚基的内消旋-A2pm残基的氨基相连。内消旋二氨基庚二酸的所有L-氨基都参与交联。给出了解乳糖优杆菌细胞壁肽聚糖肽部分的可能结构。
