Riddle V G, Harris H
J Cell Sci. 1976 Oct;22(1):199-215. doi: 10.1242/jcs.22.1.199.
Rat hepatoma cells were fused with cells of an established mouse lymphoma line, with normal diploid mouse macrophages, lymphocytes and fibroblasts and with normal diploid rat macrophages and lymphocytes. The liver-specific enzyme tyrosine aminotransferase was produced by almost all the hybrid cells, but usually at a lower level than in the parental hepatoma cells. Most of the hybrids also showed increased levels of this enzyme after exposure to dexamethasone. In the rat x mouse hybrids, the electrophoretic mobility of the enzyme indicated that only the rat hepatoma enzyme was produced. The findings are difficult to explain in terms of simple models involving a single diffusible repressor or activator of tyrosine aminotransferase synthesis.
将大鼠肝癌细胞与一个已建立的小鼠淋巴瘤细胞系的细胞、正常二倍体小鼠巨噬细胞、淋巴细胞和成纤维细胞以及正常二倍体大鼠巨噬细胞和淋巴细胞进行融合。几乎所有杂交细胞都产生了肝脏特异性酶酪氨酸转氨酶，但通常其水平低于亲代肝癌细胞。大多数杂交细胞在暴露于地塞米松后，该酶水平也有所升高。在大鼠与小鼠的杂交细胞中，该酶的电泳迁移率表明只产生了大鼠肝癌酶。这些发现很难用涉及酪氨酸转氨酶合成的单一可扩散阻遏物或激活物的简单模型来解释。
