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培养的肝癌细胞无细胞提取物中诱导型酪氨酸转氨酶的合成。

Synthesis of inducible tyrosine aminotransferase in a cell-free extract from cultured hepatoma cells.

作者信息

Beck J P, Beck G, Wong K Y, Tomkins G M

出版信息

Proc Natl Acad Sci U S A. 1972 Dec;69(12):3615-9. doi: 10.1073/pnas.69.12.3615.

DOI:10.1073/pnas.69.12.3615
PMID:4405028
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC389833/
Abstract

A cell-free extract from cultured rat hepatoma cells is described that incorporates amino acids into chains of tyrosine aminotransferase that are identical, by several criteria, with tyrosine aminotransferase from rat liver. Extracts from steroid-induced cells are 6-to 10-times more active for tyrosine aminotransferase synthesis than extracts from uninduced cells, although the total incorporation rates are identical.

摘要

本文描述了一种从培养的大鼠肝癌细胞中提取的无细胞提取物，该提取物能将氨基酸掺入酪氨酸转氨酶链中，经多项标准鉴定，这些链与大鼠肝脏中的酪氨酸转氨酶相同。来自类固醇诱导细胞的提取物在合成酪氨酸转氨酶方面的活性比未诱导细胞的提取物高6至10倍，尽管总的掺入率相同。

相似文献

1
Synthesis of inducible tyrosine aminotransferase in a cell-free extract from cultured hepatoma cells.培养的肝癌细胞无细胞提取物中诱导型酪氨酸转氨酶的合成。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3615-9. doi: 10.1073/pnas.69.12.3615.
2
Further evidence for posttranscriptional control of inducible tyrosine aminotransferase synthesis in cultured hepatoma cells.培养的肝癌细胞中诱导型酪氨酸转氨酶合成的转录后调控的进一步证据。
Nat New Biol. 1972 Sep 6;239(88):9-14. doi: 10.1038/newbio239009a0.
3
Dexamethasone phosphate-induced synthesis of tyrosine aminotransferase in hepatoma tissue culture cells. Studies of the early phases of induction and of the steroid requirement for maintanance of the induced rate of synthesis.磷酸地塞米松诱导肝癌组织培养细胞中酪氨酸转氨酶的合成。诱导早期阶段及维持诱导合成速率所需类固醇的研究。
J Biol Chem. 1970 Mar 25;245(6):1472-8.
4
Inhibition of steroid inducible tyrosine aminotransferase by mouse and rat interferon in hepatoma tissue culture cells.小鼠和大鼠干扰素对肝癌组织培养细胞中类固醇诱导的酪氨酸转氨酶的抑制作用。
FEBS Lett. 1974 Nov 15;48(2):297-300. doi: 10.1016/0014-5793(74)80490-4.
5
Stimulation of tyrosine aminotransferase synthesis by dexamethasone phosphate in cell culture.磷酸地塞米松在细胞培养中对酪氨酸转氨酶合成的刺激作用。
J Mol Biol. 1968 Jul 28;35(2):291-301. doi: 10.1016/s0022-2836(68)80025-7.
6
Mechanism of hormonal induction of tyrosine aminotransferase studied by measurement of the concentration of growing enzyme molecules.通过测量生长中酶分子的浓度研究激素诱导酪氨酸转氨酶的机制。
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2937-41. doi: 10.1073/pnas.69.10.2937.
7
Kinetics of steroid induction and deinduction of tyrosine aminotransferase synthesis in cultured hepatoma cells.培养的肝癌细胞中酪氨酸转氨酶合成的类固醇诱导和去诱导动力学
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2007-11. doi: 10.1073/pnas.72.6.2007.
8
Control of degradation and synthesis of induced tyrosine aminotransferase studied in hepatoma cells in culture.在培养的肝癌细胞中研究诱导型酪氨酸转氨酶降解与合成的调控。
Nature. 1969 Nov 22;224(5221):806-8. doi: 10.1038/224806b0.
9
The role of transcortin in glucocorticoid mediated enzyme induction: tyrosine aminotransferase induction in hepatoma tissue culture cells.皮质素运载蛋白在糖皮质激素介导的酶诱导中的作用：肝癌组织培养细胞中酪氨酸转氨酶的诱导
J Steroid Biochem. 1974 Aug;5(5):461-5. doi: 10.1016/0022-4731(74)90044-2.
10
Interconversion of multiple forms of tyrosine aminotransferase in vitro and in vivo in cultured hepatoma cells.培养的肝癌细胞中酪氨酸转氨酶多种形式在体外和体内的相互转化
Biochim Biophys Acta. 1977 Jun 23;498(1):83-90. doi: 10.1016/0304-4165(77)90089-7.

引用本文的文献

1
Regulation of the synthesis of tyrosine aminotransferase: the relationship to mRNATAT.酪氨酸转氨酶合成的调节：与mRNA TAT的关系。
Mol Cell Biochem. 1983;53-54(1-2):113-28. doi: 10.1007/BF00225249.
2
Messenger RNA for hepatic tryptophan oxygenase: its partial purification, its translation in a heterologous cell-free system, and its control by glucocorticoid hormones.肝脏色氨酸加氧酶信使核糖核酸：其部分纯化、在异源无细胞系统中的翻译以及糖皮质激素对其的调控。
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1218-21. doi: 10.1073/pnas.70.4.1218.
3
Specific changes in the messenger ribonucleic acid content of the rat ventral prostate gland after androgenic stimulation. Evidence from the synthesis of aldolase messenger ribonucleic acid.雄激素刺激后大鼠腹侧前列腺信使核糖核酸含量的特异性变化。来自醛缩酶信使核糖核酸合成的证据。
Biochem J. 1974 Nov;144(2):413-26. doi: 10.1042/bj1440413.
4
Synthesis of phosphoenolpyruvate carboxykinase (guanosine triphosphate) by isolated liver polyribosomes.分离的肝脏多核糖体合成磷酸烯醇丙酮酸羧激酶（三磷酸鸟苷）
Biochem J. 1974 Nov;144(2):199-207. doi: 10.1042/bj1440199.
5
Rabbit beta-glucuronidase. Subcellular distribution and immunochemical properties.兔β-葡萄糖醛酸酶。亚细胞分布及免疫化学特性。
Biochem J. 1974 Mar;138(3):407-13. doi: 10.1042/bj1380407.
6
Synthesis of delta-aminolaevulinate synthase by isolated liver polyribosomes.分离的肝脏多核糖体合成δ-氨基乙酰丙酸合酶。
Biochem J. 1976 Aug 15;158(2):391-400. doi: 10.1042/bj1580391.
7
Kinetics of steroid induction and deinduction of tyrosine aminotransferase synthesis in cultured hepatoma cells.培养的肝癌细胞中酪氨酸转氨酶合成的类固醇诱导和去诱导动力学
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2007-11. doi: 10.1073/pnas.72.6.2007.
8
Evidence for acceleration of the rate of elongation of tyrosine aminotransferase nascent chains by dibutyryl cyclic AMP.二丁酰环磷酸腺苷加速酪氨酸转氨酶新生链延伸速率的证据。
Proc Natl Acad Sci U S A. 1978 Jan;75(1):140-4. doi: 10.1073/pnas.75.1.140.
9
Differential degradation of messenger RNAs in mammalian cells.哺乳动物细胞中信使核糖核酸的差异降解
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2634-8. doi: 10.1073/pnas.73.8.2634.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Adaptation of the rat liver tyrosine-alpha-ketoglutarate transaminase.大鼠肝脏酪氨酸-α-酮戊二酸转氨酶的适应性
Biochim Biophys Acta. 1957 Oct;26(1):85-8. doi: 10.1016/0006-3002(57)90057-4.
3
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
4
Studies on the degradation of tyrosine aminotransferase in hepatoma cells in culture. Influence of the composition of the medium and adenosine triphosphate dependence.培养的肝癌细胞中酪氨酸转氨酶降解的研究。培养基成分的影响及三磷酸腺苷依赖性
J Biol Chem. 1971 Feb 10;246(3):710-4.
5
Specific enzyme production in eukaryotic cells.真核细胞中的特定酶产生
Adv Cell Biol. 1971;2:299-322. doi: 10.1007/978-1-4615-9588-5_6.
6
Role of DNA and specific cytoplasmic receptors in glucocorticoid action.DNA和特定细胞质受体在糖皮质激素作用中的作用。
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1892-6. doi: 10.1073/pnas.69.7.1892.
7
Glucocorticoid receptors: relations between steroid binding and biological effects.糖皮质激素受体：类固醇结合与生物学效应之间的关系。
J Mol Biol. 1972 Jun 14;67(1):99-115. doi: 10.1016/0022-2836(72)90389-0.
8
Stimulation of tyrosine aminotransferase synthesis by dexamethasone phosphate in cell culture.磷酸地塞米松在细胞培养中对酪氨酸转氨酶合成的刺激作用。
J Mol Biol. 1968 Jul 28;35(2):291-301. doi: 10.1016/s0022-2836(68)80025-7.
9
Partial purification by affinity chromatography of tyrosine aminotransferase-synthesizing ribosomes from hepatoma tissue culture cells.通过亲和层析对肝癌组织培养细胞中合成酪氨酸转氨酶的核糖体进行部分纯化。
Proc Natl Acad Sci U S A. 1971 May;68(5):1014-8. doi: 10.1073/pnas.68.5.1014.
10
Purification and properties of rat liver tyrosine aminotransferase.大鼠肝脏酪氨酸转氨酶的纯化及性质
J Biol Chem. 1969 Jul 10;244(13):3618-24.