培养的肝癌细胞无细胞提取物中诱导型酪氨酸转氨酶的合成。
Synthesis of inducible tyrosine aminotransferase in a cell-free extract from cultured hepatoma cells.
作者信息
Beck J P, Beck G, Wong K Y, Tomkins G M
出版信息
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3615-9. doi: 10.1073/pnas.69.12.3615.
Abstract
A cell-free extract from cultured rat hepatoma cells is described that incorporates amino acids into chains of tyrosine aminotransferase that are identical, by several criteria, with tyrosine aminotransferase from rat liver. Extracts from steroid-induced cells are 6-to 10-times more active for tyrosine aminotransferase synthesis than extracts from uninduced cells, although the total incorporation rates are identical.
摘要
本文描述了一种从培养的大鼠肝癌细胞中提取的无细胞提取物，该提取物能将氨基酸掺入酪氨酸转氨酶链中，经多项标准鉴定，这些链与大鼠肝脏中的酪氨酸转氨酶相同。来自类固醇诱导细胞的提取物在合成酪氨酸转氨酶方面的活性比未诱导细胞的提取物高6至10倍，尽管总的掺入率相同。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
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