Vanlić-Razumenić N M, Gorkić D A
Eur J Nucl Med. 1976 Dec 30;1(4):235-42. doi: 10.1007/BF00252169.
Studies of the chemistry of preparation of tin chelate 2.3-dimercaptosuccinic acid (DMS) complexed with 99mTc with respect to its biological properties have been made. We have varied molar ratio of reactants (DMS:Sn/II/), concentration of Sn-DMS chelate and pH of 99mTc-DMS injection solution and examined their influence on biodistribution. Biodynamics of labeled solution at time intervals of from 0.5 to 4 h have also been measured. Animal experiments were carried out on mice and rats. Radiochemical purity was checked by paper chromatography, thin-layer chromatography, and gel-filtration through Bio-Gel P-2. A sterile, pyrogen-free lyophilized "kit" for labeling, stable for 6 months, was made on the basis of achieved results. The results of its clinical application are also reported.
针对与99mTc络合的锡螯合物2,3-二巯基丁二酸（DMS）制备化学的生物学特性进行了研究。我们改变了反应物的摩尔比（DMS:Sn/II/）、Sn-DMS螯合物的浓度以及99mTc-DMS注射溶液的pH值，并研究了它们对生物分布的影响。还测量了标记溶液在0.5至4小时时间间隔内的生物动力学。在小鼠和大鼠身上进行了动物实验。通过纸色谱法、薄层色谱法以及通过Bio-Gel P-2进行凝胶过滤来检查放射化学纯度。基于所取得的结果，制备了一种无菌、无热原的冻干“试剂盒”，其稳定性可达6个月。还报告了其临床应用结果。
