Vanlić-Razumenić N
Nuklearmedizin. 1980 Feb;19(1):37-40.
The chemical and biological properties of two freeze-dried DMS kits for labelling with 99mTc (Tc P-5, manufactured by Boris Kidric Institute, Vinca, and TCK-12, by CIS, Saclay) were compared. A method for the determination of the radiochemical composition of the injection solution is proposed: ascending Whatman 31 paper chromatography in 50% aqueous methanol. The chromatographic results correspond to the biologic activity of the preparations. The biodynamics of both radiopharmaceuticals were determined at intervals in rats. Maximum renal concentrations were achieved at 3-4 hrs post injection (52% kidney and 3% liver uptake for Tc P-5; 59% kidney and 6-8% liver uptake for TCK-12). The bench-lifes of the injection solutions were compared by determining organ distribution after standing of the solutions for 0, 2 and 4 hrs. The Tc P-5 preparation was more stable, since the renal and hepatic concentrations were nearly constant. On the other hand, the hepatic concentration of TCK-12 was increasing during aging of the solution with a simultaneous decrease in renal concentration.
比较了两种用于用99mTc标记的冻干二甲基亚砜（DMS）试剂盒（由温察的鲍里斯·基德里克研究所生产的Tc P - 5和由萨克莱的CIS生产的TCK - 12）的化学和生物学性质。提出了一种测定注射溶液放射化学组成的方法：在50%甲醇水溶液中进行Whatman 31号纸上层析。色谱结果与制剂的生物活性相对应。在大鼠体内定期测定了两种放射性药物的生物动力学。注射后3 - 4小时达到最大肾脏浓度（Tc P - 5为肾脏摄取52%，肝脏摄取3%；TCK - 12为肾脏摄取59%，肝脏摄取6 - 8%）。通过测定溶液静置0、2和4小时后的器官分布来比较注射溶液的有效期。Tc P - 5制剂更稳定，因为肾脏和肝脏浓度几乎保持不变。另一方面，TCK - 12溶液在老化过程中肝脏浓度增加，同时肾脏浓度降低。
