Bomsel-Helmreich O, Papiernik-Berkhauer E
Acta Genet Med Gemellol (Roma). 1976;25:73-6. doi: 10.1017/s000156600001388x.
Aiming to show that delayed ovulation may induce MZ twinning, follicular maturation was induced in the rabbit by a small quantity of Pregnant Mare Serum Gonadotropin (16 UI four times): coitus, which induces ovulation in the rabbit, was delayed 60 hours after the last injection. From the 387 blastocysts obtained after this treatment, 6 (1.5%) were pairs of MZ twins, twinning being otherwise exceptional in the rabbit. Other anomalies were shown by the embryos, apparently related to a deficient quality of the eggs: high embryonic mortality (62% vs. 27% in controls) and chromosomal anomalies (20%) such as trisomies, triploidies, and chimaeras. The relation between MZ twinning, chromosomal anomalies, and embryonic mortality induced by delayed ovulation, could be connected and related to the poor perinatal conditions frequently observed in human MZ twins.
为了证明排卵延迟可能会导致单合子双胞胎的形成，给兔子注射少量孕马血清促性腺激素（16国际单位，共四次）来诱导卵泡成熟：在兔子中诱导排卵的交配在最后一次注射后延迟60小时。在这种处理后获得的387个囊胚中，有6个（1.5%）是单合子双胞胎对，而在兔子中双胞胎现象原本是很罕见的。胚胎还表现出其他异常情况，显然与卵子质量欠佳有关：胚胎死亡率很高（62%，而对照组为27%）以及染色体异常（20%），如三体性、三倍体和嵌合体。由排卵延迟引起的单合子双胞胎、染色体异常和胚胎死亡之间的关系，可能与人类单合子双胞胎中经常观察到的不良围产期状况有关联。
