一种来自豆科植物根的具有腺苷三磷酸双磷酸酶活性的结瘤因子结合凝集素。
A nod factor binding lectin with apyrase activity from legume roots.
作者信息
Etzler M E, Kalsi G, Ewing N N, Roberts N J, Day R B, Murphy J B
机构信息
Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 May 11;96(10):5856-61. doi: 10.1073/pnas.96.10.5856.
Abstract
A lectin isolated from the roots of the legume, Dolichos biflorus, binds to Nod factors produced by rhizobial strains that nodulate this plant and has a deduced amino acid sequence with no significant homology to any lectin reported to date. This lectin also is an enzyme that catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside di- and triphosphates; the enzyme activity is increased in the presence of carbohydrate ligands. This lectin-nucleotide phosphohydrolase (LNP) has a substrate specificity characteristic of the apyrase category of phosphohydrolases, and its sequence contains four motifs characteristic of this category of enzymes. LNP is present on the surface of the root hairs, and treatment of roots with antiserum to LNP inhibits their ability to undergo root hair deformation and to form nodules on exposure to rhizobia. These properties suggest that this protein may play a role in the rhizobium-legume symbiosis and/or in a related carbohydrate recognition event endogenous to the plant.
摘要
从豆科植物双花扁豆根部分离出的一种凝集素，能与可使该植物结瘤的根瘤菌菌株产生的结瘤因子结合，其推导的氨基酸序列与迄今报道的任何凝集素均无明显同源性。这种凝集素也是一种酶，可催化核苷二磷酸和三磷酸的磷酸酐键水解；在碳水化合物配体存在的情况下，酶活性会增强。这种凝集素 - 核苷酸磷酸水解酶（LNP）具有磷酸水解酶中腺苷三磷酸双磷酸酶类别的底物特异性特征，其序列包含该类酶的四个基序。LNP存在于根毛表面，用抗LNP血清处理根部会抑制其在接触根瘤菌时发生根毛变形和形成根瘤的能力。这些特性表明，这种蛋白质可能在根瘤菌 - 豆科植物共生关系和/或植物内源性相关碳水化合物识别事件中发挥作用。
相似文献
1
A nod factor binding lectin with apyrase activity from legume roots.一种来自豆科植物根的具有腺苷三磷酸双磷酸酶活性的结瘤因子结合凝集素。
Proc Natl Acad Sci U S A. 1999 May 11;96(10):5856-61. doi: 10.1073/pnas.96.10.5856.
2
Localization of a Nod factor-binding protein in legume roots and factors influencing its distribution and expression.豆科植物根中一种结瘤因子结合蛋白的定位及其分布和表达的影响因素
Plant Physiol. 2000 Nov;124(3):1039-48. doi: 10.1104/pp.124.3.1039.
3
A Nod factor-binding lectin is a member of a distinct class of apyrases that may be unique to the legumes.一种结瘤因子结合凝集素是一类独特的焦磷酸酶的成员，这类焦磷酸酶可能是豆科植物所特有的。
Mol Gen Genet. 1999 Sep;262(2):261-7. doi: 10.1007/s004380051082.
4
Heterologous rhizobial lipochitin oligosaccharides and chitin oligomers induce cortical cell divisions in red clover roots, transformed with the pea lectin gene.异源根瘤菌脂壳寡糖和壳寡糖可诱导用豌豆凝集素基因转化的红三叶草根中的皮层细胞分裂。
Mol Plant Microbe Interact. 2000 Mar;13(3):268-76. doi: 10.1094/MPMI.2000.13.3.268.
5
Role of lectins (and rhizobial exopolysaccharides) in legume nodulation.
Curr Opin Plant Biol. 1999 Aug;2(4):320-6. doi: 10.1016/S1369-5266(99)80056-9.
6
7
Binding of isolated plant lectin by rhizobia during episodes of reduced gravity obtained by parabolic flight.在抛物线飞行获得的微重力条件下，根瘤菌对分离出的植物凝集素的结合。
Plant Physiol. 1990;92(1):262-4. doi: 10.1104/pp.92.1.262.
8
Nod factors of Rhizobium are a key to the legume door.根瘤菌的结瘤因子是打开豆科植物大门的钥匙。
Mol Microbiol. 1994 Jul;13(1):171-8. doi: 10.1111/j.1365-2958.1994.tb00412.x.
9
Purification and characterization of a membrane-bound ATP diphosphohydrolase from Cicer arietinum (chick-pea)roots.来自鹰嘴豆（鸡豆）根的膜结合ATP二磷酸水解酶的纯化与特性分析
Biochem J. 1981 Sep 1;197(3):637-43. doi: 10.1042/bj1970637.
10
Weak protein-protein interactions in lectins: the crystal structure of a vegetative lectin from the legume Dolichos biflorus.凝集素中弱的蛋白质-蛋白质相互作用：豆科植物双花扁豆营养期凝集素的晶体结构
J Mol Biol. 2001 May 25;309(1):193-201. doi: 10.1006/jmbi.2001.4639.
引用本文的文献
1
Apyrase in horticultural crops: insights into growth, stress adaptation and quality regulation.园艺作物中的三磷酸腺苷双磷酸酶：对生长、胁迫适应和品质调控的见解
Mol Hortic. 2025 Sep 1;5(1):40. doi: 10.1186/s43897-025-00168-w.
2
Nitrogen fixation in maize: breeding opportunities.玉米的固氮作用：育种机会。
Theor Appl Genet. 2021 May;134(5):1263-1280. doi: 10.1007/s00122-021-03791-5. Epub 2021 Mar 7.
3
Structure-function and application of plant lectins in disease biology and immunity.植物凝集素的结构-功能及其在疾病生物学和免疫中的应用。
Food Chem Toxicol. 2019 Dec;134:110827. doi: 10.1016/j.fct.2019.110827. Epub 2019 Sep 19.
4
A cytoplasmic kinase connects Nod factor perception by the NFR5 LysM receptor to nodulation.细胞质激酶将 NFR5LysM 受体对 Nod 因子的感知与结瘤连接起来。
Proc Natl Acad Sci U S A. 2019 Jul 9;116(28):14339-14348. doi: 10.1073/pnas.1815425116. Epub 2019 Jun 25.
5
Structures and kinetics for plant nucleoside triphosphate diphosphohydrolases support a domain motion catalytic mechanism.植物核苷三磷酸二磷酸水解酶的结构与动力学支持结构域运动催化机制。
Protein Sci. 2017 Aug;26(8):1627-1638. doi: 10.1002/pro.3199. Epub 2017 Jun 6.
6
Lipo-chitooligosaccharidic nodulation factors and their perception by plant receptors.脂壳寡糖结瘤因子及其被植物受体识别
Glycoconj J. 2015 Oct;32(7):455-64. doi: 10.1007/s10719-015-9609-3. Epub 2015 Aug 2.
7
Populus euphratica APYRASE2 Enhances Cold Tolerance by Modulating Vesicular Trafficking and Extracellular ATP in Arabidopsis Plants.胡杨APYRASE2通过调节拟南芥植物中的囊泡运输和细胞外ATP来增强耐寒性。
Plant Physiol. 2015 Sep;169(1):530-48. doi: 10.1104/pp.15.00581. Epub 2015 Jul 29.
8
Extracellular ATP acts as a damage-associated molecular pattern (DAMP) signal in plants.细胞外ATP在植物中作为一种损伤相关分子模式（DAMP）信号发挥作用。
Front Plant Sci. 2014 Sep 3;5:446. doi: 10.3389/fpls.2014.00446. eCollection 2014.
9
The nodulation factor hydrolase of Medicago truncatula: characterization of an enzyme specifically cleaving rhizobial nodulation signals.蒺藜苜蓿结瘤因子水解酶：一种特异性切割根瘤菌结瘤信号的酶的特性研究。
Plant Physiol. 2013 Nov;163(3):1179-90. doi: 10.1104/pp.113.223966. Epub 2013 Sep 30.
10
Rhizobial and mycorrhizal symbioses in Lotus japonicus require lectin nucleotide phosphohydrolase, which acts upstream of calcium signaling.在百脉根中，根瘤菌和菌根共生需要凝集素核苷酸磷酸水解酶，它在钙信号的上游起作用。
Plant Physiol. 2013 Jan;161(1):556-67. doi: 10.1104/pp.112.206110. Epub 2012 Nov 7.
本文引用的文献
1
Symbiotic Nitrogen Fixation.共生固氮作用
Plant Cell. 1995 Jul;7(7):869-885. doi: 10.1105/tpc.7.7.869.
2
The transmembrane domains of ectoapyrase (CD39) affect its enzymatic activity and quaternary structure.胞外三磷酸腺苷双磷酸酶（CD39）的跨膜结构域影响其酶活性和四级结构。
J Biol Chem. 1998 Sep 18;273(38):24814-21. doi: 10.1074/jbc.273.38.24814.
3
Lotus corniculatus nodulation specificity is changed by the presence of a soybean lectin gene.百脉根的结瘤特异性因大豆凝集素基因的存在而改变。
Plant Cell. 1998 Aug;10(8):1233-50. doi: 10.1105/tpc.10.8.1233.
4
Purification and properties of a basic endo-1,3-beta-glucanase from rice (Oryza sativa L.).水稻（Oryza sativa L.）中一种碱性内切-1,3-β-葡聚糖酶的纯化及性质
Plant Cell Physiol. 1996 Jul;37(5):702-5. doi: 10.1093/oxfordjournals.pcp.a029002.
5
Rhizobium lipo-chitooligosaccharide nodulation factors: signaling molecules mediating recognition and morphogenesis.根瘤菌脂壳寡糖结瘤因子：介导识别与形态发生的信号分子
Annu Rev Biochem. 1996;65:503-35. doi: 10.1146/annurev.bi.65.070196.002443.
6
Structural determination of symbiotic nodulation factors from the broad host-range Rhizobium species NGR234.来自广宿主根瘤菌NGR234的共生结瘤因子的结构测定
Carbohydr Res. 1996 Aug 19;289:115-36. doi: 10.1016/0008-6215(96)00119-x.
7
Characterization of an Arabidopsis thaliana gene that defines a new class of putative plant receptor kinases with an extracellular lectin-like domain.对一个拟南芥基因的表征，该基因定义了一类具有细胞外凝集素样结构域的新型假定植物受体激酶。
J Mol Biol. 1996 May 24;258(5):778-88. doi: 10.1006/jmbi.1996.0286.
8
Light-modulated abundance of an mRNA encoding a calmodulin-regulated, chromatin-associated NTPase in pea.豌豆中一种编码受钙调蛋白调节的、与染色质相关的NTPase的mRNA的光调控丰度。
Plant Mol Biol. 1996 Jan;30(1):135-47. doi: 10.1007/BF00017808.
9
Photometric microtiter assay of inorganic phosphate in the presence of acid-labile organic phosphates.在存在酸不稳定有机磷酸盐的情况下对无机磷酸盐进行光度微量滴定测定。
Anal Biochem. 1995 Sep 1;230(1):173-7. doi: 10.1006/abio.1995.1453.
10
Purification and cloning of a soluble ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).从马铃薯块茎（茄属马铃薯）中纯化和克隆一种可溶性ATP二磷酸水解酶（腺苷三磷酸双磷酸酶）
Biochem Biophys Res Commun. 1996 Jan 26;218(3):916-23. doi: 10.1006/bbrc.1996.0162.
Zotero 插件