Danfour M, Schorah C J, Evans S W
Molecular Epidemiology Unit, Research School of Medicine, University of Leeds, UK.
Immunopharmacol Immunotoxicol. 1999 May;21(2):277-93. doi: 10.3109/08923979909052763.
Glutathione (GSH) concentrations have been determined in the human myeloid cell line U937. The effects of modulating GSH concentration on sensitivity to metal toxicity has also been examined. Intracellular concentrations of GSH increased as the cells entered into the cell cycle, reaching a maximum level after 24 hours of cell culture, after which levels declined. Cell concentration was also observed to influence intracellular GSH concentrations. A reciprocal relationship was observed with higher maximum intracellular GSH concentrations being measured in cultures initiated with smaller cell number. The relative toxicity's determined for five metal chlorides were mercury > cadmium > cobalt > zinc > gold. Treatment of cells with N-acetylcysteine (NAC) increased intracellular GSH but had little effect on the absolute or relative toxicity's of the metals. Treatment of cells with L-buthionine-(S-R)-sulfoximine (BSO) depleted intracellular GSH and resulted in increased sensitivity of the cells to gold, 40 fold, cadmium 8 fold and mercury 3 fold.
已测定人髓样细胞系U937中的谷胱甘肽（GSH）浓度。还研究了调节GSH浓度对金属毒性敏感性的影响。随着细胞进入细胞周期，细胞内GSH浓度升高，在细胞培养24小时后达到最高水平，之后浓度下降。还观察到细胞浓度会影响细胞内GSH浓度。观察到一种反比关系，即接种细胞数量较少的培养物中测得的细胞内GSH最高浓度较高。测定了五种金属氯化物的相对毒性，结果为汞＞镉＞钴＞锌＞金。用N-乙酰半胱氨酸（NAC）处理细胞可增加细胞内GSH，但对金属的绝对或相对毒性影响不大。用L-丁硫氨酸-（S-R）-亚砜亚胺（BSO）处理细胞可耗尽细胞内GSH，并导致细胞对金的敏感性增加40倍，对镉的敏感性增加8倍，对汞的敏感性增加3倍。
