Capodicasa E, De Bellis F, Pelli M A
Institute of Internal Medicine and Oncological Sciences, Perugia University, Italy.
Immunopharmacol Immunotoxicol. 1999 May;21(2):357-77. doi: 10.3109/08923979909052768.
Recent findings on the capacity of omeprazole to influence various leukocyte functions, in vitro, raises the question on the potential use of protonic pump inhibitors, commonly used in the treatment of acid-secretion-related disorders, as immunomodulators. The aim of this study was to evaluate the in vitro effect of lansoprazole on human natural killer (NK) cell cytotoxix activity, chemotaxis and superoxide anion (O2*-) generation exerted by polymorphonucleated cells (PMNs). NK cytotoxicity activity was assessed by a 51Cr release assay, PMN chemotaxis was determined by an under agarose method and O2*- generation was analyzed on the basis of reduced cytochrome C. Incubation times with lansoprazole was 30 min for PMNs and 1-4.5 hours for NK cells, respectively. Lansoprazole induced significant dose dependent inhibition of NK cell activity and PMN functions at concentrations ranging from 100 to 1,000 microM. This study demonstrate that lansoprazole, like omeprazole, inhibits several leukocyte functions, in vitro, then suggesting that protonic pump inhibitors are able to provoke these effects, at least at certain doses.
近期关于奥美拉唑在体外影响各种白细胞功能的研究结果，引发了对于常用于治疗酸分泌相关疾病的质子泵抑制剂作为免疫调节剂潜在用途的质疑。本研究的目的是评估兰索拉唑对人自然杀伤（NK）细胞细胞毒性活性、趋化性以及多形核细胞（PMN）产生超氧阴离子（O2*-）的体外作用。通过51Cr释放试验评估NK细胞毒性活性，采用琼脂糖下法测定PMN趋化性，并基于细胞色素C还原分析O2*-的产生。与兰索拉唑的孵育时间，PMN为30分钟，NK细胞为1至4.5小时。兰索拉唑在100至1000微摩尔浓度范围内可显著剂量依赖性抑制NK细胞活性和PMN功能。本研究表明，兰索拉唑与奥美拉唑一样，在体外可抑制多种白细胞功能，这表明质子泵抑制剂至少在某些剂量下能够引发这些效应。
