Clp 依赖 ATP 的蛋白酶合成的改变影响链霉菌的形态和生理分化。
Alteration of the synthesis of the Clp ATP-dependent protease affects morphological and physiological differentiation in Streptomyces.
作者信息
de Crécy-Lagard V, Servant-Moisson P, Viala J, Grandvalet C, Mazodier P
机构信息
Unité de Biochimie Microbienne, URA 1300 CNRS, Institut Pasteur, 28 rue du Dr Roux, Paris cedex 75724, France.
出版信息
Mol Microbiol. 1999 May;32(3):505-17. doi: 10.1046/j.1365-2958.1999.01364.x.
The genes of Streptomyces coelicolor A3(2) encoding catalytic subunits (ClpP) and regulatory subunits (ClpX and ClpC) of the ATP-dependent protease family Clp were cloned, mapped and characterized. S. coelicolor contains at least two clpP genes, clpP1 and clpP2, located in tandem upstream from the clpX gene, and at least two unlinked clpC genes. Disruption of the clpP1 gene in S. lividans and S. coelicolor blocks differentiation at the substrate mycelium step. Overexpression of clpP1 and clpP2 accelerates aerial mycelium formation in S. lividans, S. albus and S. coelicolor. Overproduction of ClpX accelerates actinorhodin production in S. coelicolor and activates its production in S. lividans.
对天蓝色链霉菌A3(2)中编码ATP依赖性蛋白酶家族Clp的催化亚基（ClpP）和调节亚基（ClpX和ClpC）的基因进行了克隆、定位和表征。天蓝色链霉菌含有至少两个clpP基因，即clpP1和clpP2，它们串联位于clpX基因上游，以及至少两个不连锁的clpC基因。在变铅青链霉菌和天蓝色链霉菌中破坏clpP1基因会在底物菌丝体阶段阻断分化。clpP1和clpP2的过表达会加速变铅青链霉菌、白色链霉菌和天蓝色链霉菌中气生菌丝的形成。ClpX的过量产生会加速天蓝色链霉菌中放线紫红素的产生，并在变铅青链霉菌中激活其产生。
Zotero 插件