Yang X, Zhuang Z, Tan B
School of Public Health, Sun Yat-sen University of Medical Sciences, Guangzhou.
Zhonghua Yu Fang Yi Xue Za Zhi. 1998 May;32(3):147-9.
To study the effects of carbon disulfide on adrenocortical function and analyze and compare the various biological materials used in biological monitoring by observing the changes of adrenocortical hormones and their metabolites in blood, saliva and urine of a viscose rayon mill exposed to carbon disulfide for long-term.
Cortisol in blood and saliva was determined by 125I-radioimmunoassay (RIA), cortisol and cortisone in urine by high performance liquid chromatogaphy (HPLC), and their metabolite 17-ketosteroid by chemical method. Data collected were analyzed by SPSS software in microcomputer to compare their difference between two groups, relationship between them and concentrations of carbon disulfide, and consistence between nontraumatic indices in saliva and urine and blood hormone.
Levels of adrenocortical hormones and their metabolites in blood, urine and saliva decreased in the workers exposed to concentrations of carbon disulfide exceeding the national allowable standard, and correlated closely with them. There was a good correlationship between indices in blood and in urine.
Blood cortisol, urine free cortisol, cortisone and its metabolite 17-ketosteroid all are good indices for biological effects reflecting adrenocortical function, and detection for urine cortisol and its metabolite is more practicable because of its non-traumatic features.
通过观察长期接触二硫化碳的粘胶纤维厂工人血液、唾液和尿液中肾上腺皮质激素及其代谢产物的变化，研究二硫化碳对肾上腺皮质功能的影响，并分析比较生物监测中所采用的各种生物样品。
采用125I放射免疫分析法（RIA）测定血液和唾液中的皮质醇，高效液相色谱法（HPLC）测定尿液中的皮质醇和可的松，化学法测定其代谢产物17-酮类固醇。用微机SPSS软件对所收集的数据进行分析，比较两组间的差异、它们之间的关系以及与二硫化碳浓度的关系，同时比较唾液和尿液中无创指标与血液激素之间的一致性。
接触二硫化碳浓度超过国家允许标准的工人，其血液、尿液和唾液中肾上腺皮质激素及其代谢产物水平均下降，并与二硫化碳浓度密切相关。血液和尿液中的指标之间存在良好的相关性。
血液皮质醇、尿游离皮质醇、可的松及其代谢产物17-酮类固醇均是反映肾上腺皮质功能生物效应的良好指标，而尿皮质醇及其代谢产物检测因无创而更具实用性。
