Dahmoun M, Boman K, Cajander S, Westin P, Bäckström T
Department of Obstetrics and Gynecology and Mid Sweden Research and Development Center, Sundsvall Hospital, University of Umea, Sweden.
J Clin Endocrinol Metab. 1999 May;84(5):1737-43. doi: 10.1210/jcem.84.5.5706.
Apoptosis with one regulator, Bcl-2, and proliferation with the marker Ki-67 were studied in 75 endometrial biopsies representing superficial parts of endometrium from 35 regularly menstruating women premenstrually and menstrually. Hormonal withdrawal was studied in serum samples and potentiated in epithelium by the decreasing 17beta-estradiol and progesterone receptor scores 4 days premenstrually. The apoptotic index increased 2 days before the onset of menstruation and peaked on the second menstrual day. The high apoptotic index together with low proliferation in endometrial epithelium at the end of the menstrual cycle are similar to the involution process seen in other hormone-dependent organs. In stroma, the apoptotic index increased later, at the onset of menstruation, and the increase was lower than that in epithelium. The Ki-67 index increased during the last 3 days of the secretory phase, parallel with an increasing progesterone receptor score and decreasing Bcl-2 staining, and peaked at the onset of menstruation. The findings in stroma concur with high proliferation at the end of the menstrual cycle and high cell turnover during menstruation, suggesting the participation of stroma in the renewal process of endometrium.
在来自35名月经规律女性的75份子宫内膜活检样本中，研究了具有一种调节因子Bcl-2的细胞凋亡以及用标记物Ki-67检测的增殖情况，这些活检样本代表了月经前和月经期子宫内膜的表层部分。在血清样本中研究了激素撤退情况，并通过在月经前4天降低17β-雌二醇和孕激素受体评分来增强上皮细胞中的激素撤退。细胞凋亡指数在月经开始前2天增加，并在月经第二天达到峰值。月经周期末期子宫内膜上皮细胞中高细胞凋亡指数与低增殖情况类似于在其他激素依赖性器官中观察到的退化过程。在基质中，细胞凋亡指数在月经开始时稍后增加，且增加幅度低于上皮细胞。Ki-67指数在分泌期的最后3天增加，与孕激素受体评分增加和Bcl-2染色减少平行，并在月经开始时达到峰值。基质中的这些发现与月经周期末期的高增殖以及月经期间的高细胞更新率一致，表明基质参与了子宫内膜的更新过程。
