Unicomb L E, Podder G, Gentsch J R, Woods P A, Hasan K Z, Faruque A S, Albert M J, Glass R I
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.
J Clin Microbiol. 1999 Jun;37(6):1885-91. doi: 10.1128/JCM.37.6.1885-1891.1999.
We characterized 1,534 rotavirus (RV) strains collected in Bangladesh from 1992 to 1997 to assess temporal changes in G type and to study the most common G and P types using reverse transcription-PCR, oligonucleotide probe hybridization, and monoclonal antibody-based enzyme immunoassay. Results from this study combined with our previous findings from 1987 to 1991 (F. Bingnan et al., J. Clin. Microbiol. 29:862-868, 1991, and L. E. Unicomb et al., Arch. Virol. 132:201-208, 1993) (n = 2,515 fecal specimens) demonstrated that the distribution of the four major G types varied from year to year, types G1 to G4 constituted 51% of all strains tested (n = 1,364), and type G4 was the most prevalent type (22%), followed by type G2 (17%). Of 351 strains tested for both G and P types, three globally common types, type P[8], G1, type P[4], G2, and type P[8], G4, comprised 45% (n = 159) of the strains, although eight other strains were circulating during the study period. Mixed G and/or P types were found in 23% (n = 79) of the samples tested. Type G9 RVs that were genotype P[6] and P[8] with both long and short electrophoretic patterns emerged in 1995. The finding of five different genotypes among G9 strains, of which three were frequently detected, suggests that they may have an unusual propensity for reassortment that exceeds that found among the common G types. We also detected antigenic changes in serotypes G2 and G4 over time, as indicated by the loss of reactivity with standard typing monoclonal antibodies. Our data suggest that a vaccine must provide protection against type G9 RVs as well as against the four major G types because G9 strains constituted 16% (n = 56) of the typeable RV strains and have predominated since 1996.
我们对1992年至1997年在孟加拉国收集的1534株轮状病毒（RV）进行了特征分析，以评估G型的时间变化，并使用逆转录聚合酶链反应、寡核苷酸探针杂交和基于单克隆抗体的酶免疫测定法研究最常见的G型和P型。本研究结果与我们先前1987年至1991年的研究结果（F. Bingnan等人，《临床微生物学杂志》29:862 - 868，1991年；L. E. Unicomb等人，《病毒学档案》132:201 - 208，1993年）（n = 2515份粪便标本）相结合，表明四种主要G型的分布逐年变化，G1至G4型占所有检测菌株的51%（n = 1364），G4型是最常见的类型（22%），其次是G2型（17%）。在351株同时检测G型和P型的菌株中，三种全球常见类型，即P[8]、G1型，P[4]、G2型和P[8]、G4型，占菌株的45%（n = 159），尽管在研究期间还有其他八种菌株在传播。在23%（n = 79）的检测样本中发现了混合的G型和/或P型。1995年出现了基因型为P[6]和P[8]且具有长电泳模式和短电泳模式的G9型RV。在G9菌株中发现了五种不同的基因型，其中三种经常被检测到，这表明它们可能具有比常见G型更高的重组倾向。我们还检测到随着时间推移，G2和G4血清型的抗原性发生了变化，这表现为与标准分型单克隆抗体的反应性丧失。我们的数据表明，疫苗必须提供针对G9型RV以及四种主要G型的保护，因为G9菌株占可分型RV菌株的16%（n = 56），并且自1996年以来一直占主导地位。
