Cunliffe N A, Gondwe J S, Broadhead R L, Molyneux M E, Woods P A, Bresee J S, Glass R I, Gentsch J R, Hart C A
Department of Pediatrics, College of Medicine, University of Malawi, Blantyre.
J Med Virol. 1999 Mar;57(3):308-12.
One hundred rotavirus strains detected in children with acute diarrhea in Blantyre, Malawi, between July 1997 and January 1998 were characterized for G (VP7) and P (VP4) types by using multiplex, heminested, reverse transcription-polymerase chain reaction. A novel P[6]G8 rotavirus strain was identified in 42% of the specimens. The remaining strains comprised P[8]G3 (20%), P[6]G3 (10%), P[4]G8 (9%), P[6]G9 (3%), P[8]G4 (2%), P[6]G4 (2%), and P[4]G3 (1%). Rotavirus strains with mixed G or P types were identified in 2% of the specimens. Nine percent of the strains were nontypeable with the primers used. The P[6] genotype was identified in 57% of strains overall. This first description of serotype G8 rotavirus as a predominant strain has important implications for vaccine development in Africa. The finding of novel P/G combinations (P[6]G8 and P[4]G8) highlights the extraordinary diversity of rotaviruses in some countries.
1997年7月至1998年1月期间，在马拉维布兰太尔对100株从急性腹泻儿童中检测出的轮状病毒毒株，运用多重半套式逆转录聚合酶链反应对其G（VP7）和P（VP4）类型进行了鉴定。在42%的标本中鉴定出一种新型P[6]G8轮状病毒毒株。其余毒株包括P[8]G3（20%）、P[6]G3（10%）、P[4]G8（9%）、P[6]G9（3%）、P[8]G4（2%）、P[6]G4（2%）和P[4]G3（1%）。在2%的标本中鉴定出具有混合G或P类型的轮状病毒毒株。9%的毒株无法用所用引物进行分型。总体上在57%的毒株中鉴定出P[6]基因型。首次将血清型G8轮状病毒描述为优势毒株，这对非洲的疫苗研发具有重要意义。新型P/G组合（P[6]G8和P[4]G8）的发现凸显了某些国家轮状病毒的非凡多样性。
