Paul S K, Ahmed M U, Hossain M A, Mahmud M C, Bhuiyan M R, Saha S K, Tabassum S
Department of Microbiology, Mymensingh Medical College (MMC), Mymensingh, Bangladesh.
Mymensingh Med J. 2010 Jan;19(1):16-26.
Between July 2004 and June 2006, a total of 1438 fecal samples were collected from Hospitalized children and adults with diarrhea at an Infectious Disease Hospital (SK Hospital) in Mymensingh, Bangladesh. The diversity of rotavirus strains were investigated using electropherotyping and reverse transcription-PCR amplification of the VP7 and VP4 genes. Group A rotavirus was detected in 171 of 1438(11.9%) specimens: 13.3%(72/543) from children 6 months to </=15 years of age and 11.0%(99/895) from individuals aged >15-76 years. The electrophoretic patterns of dsRNA of rotavirus showed 11 different migrations (7 long and 4 short) by polyacrylamide gel electrophoresis (PAGE). Representative 60 positive samples were selected for PCR genotyping. For the entire period, the common rotavirus G types G1 (27%), G2 (45%), and G9 (7%), comprised 79% of the strains, and common P types, P[4] (43%), P[6] (12%), and P [8] (20%), comprised 75% of the total P types. Of note, we detected a high percentage of unusual G-type P-type combination (11%) strains and specimens with mixed (10% G and 23% P) rotavirus infection with a variety of genomic constellation. Overall, the most frequent rotavirus strains were G2P[4] (36.7%) both in children and adults which were mostly associated with VP6 genotype I (subgroup I), and NSP4 genotype A or B, followed by G1P[8] (10.0%), G9P[8] (6.7%). G12 was detected for the first time in the study area and the detection rate was G12P[6] (3.4%), and G12P[4] (1.7%). The nucleotide sequence of VP7 gene was highly homologous among G12 strains isolated in Mymensingh suggesting recent emergence from a common ancestor. The study highlighted the diversity of rotaviruses isolated from children and adults. Due to the effect of antigenic diversity on rotavirus vaccines, it is important to continue careful monitoring of these strains as rotavirus vaccine programs are being implemented in Bangladesh.
2004年7月至2006年6月期间，在孟加拉国迈门辛的一家传染病医院（SK医院），共收集了1438份腹泻住院儿童和成人的粪便样本。使用电泳分型以及VP7和VP4基因的逆转录 - PCR扩增对轮状病毒株的多样性进行了研究。在1438份样本中的171份（11.9%）中检测到A组轮状病毒：6个月至15岁儿童中的检出率为13.3%（72/543），15至76岁个体中的检出率为11.0%（99/895）。轮状病毒双链RNA的电泳图谱通过聚丙烯酰胺凝胶电泳（PAGE）显示出11种不同的迁移情况（7种长型和4种短型）。选择60份代表性阳性样本进行PCR基因分型。在整个研究期间，常见的轮状病毒G型G1（27%）、G2（45%）和G9（7%），占毒株的79%，常见的P型P[4]（43%）、P[6]（12%）和P[8]（20%），占总P型的75%。值得注意的是，我们检测到高比例的不寻常G型 - P型组合毒株（11%）以及具有多种基因组组合的混合轮状病毒感染样本（10%的G型和23%的P型）。总体而言，儿童和成人中最常见的轮状病毒株是G2P[4]（36.7%），大多与VP6基因型I（亚组I）以及NSP4基因型A或B相关，其次是G1P[8]（10.0%）和G9P[8]（6.7%）。在研究区域首次检测到G12，其检出率为G12P[6]（3.4%）和G
