Dennehy P H, Hartin M, Nelson S M, Reising S F
Division of Pediatric Infectious Diseases, Rhode Island Hospital, and Department of Pediatrics, Brown University School of Medicine, Providence, Rhode Island 02903, USA.
J Clin Microbiol. 1999 Jun;37(6):1977-9. doi: 10.1128/JCM.37.6.1977-1979.1999.
Rapid detection of group A rotavirus was performed by using ImmunoCardStat! Rotavirus (ICS-RV) (which uses immunogold-based, horizontal-flow membrane technology), two commercial enzyme immunoassays (Premier Rotaclone and TestPack Rotavirus), and electron microscopy. A total of 249 stool specimens collected from children with gastroenteritis between February and April 1997 were tested. After resolution of 19 of the 22 discordant results by reverse transcription-PCR for group A rotavirus, ICS-RV detected 125 positives while Rotaclone and TestPack detected 127 and 129 positives, respectively. The sensitivity, specificity, positive predictive value, and negative predictive value were 94.0, 100, 100, and 93.4% for ICS-RV; 95.5, 100, 100, and 95.0% for Rotaclone; and 97.0, 96.5, 97.0, and 96.5% for TestPack. ICS-RV was sensitive and specific and was relatively simple to perform and interpret.
采用免疫卡Stat!轮状病毒检测试剂盒（ICS-RV，其采用基于免疫金的水平流动膜技术）、两种商用酶免疫测定法（Premier Rotaclone和TestPack Rotavirus）以及电子显微镜对A组轮状病毒进行快速检测。对1997年2月至4月间从患肠胃炎儿童采集的249份粪便标本进行了检测。在通过A组轮状病毒逆转录聚合酶链反应解决了22个不一致结果中的19个之后，ICS-RV检测出125个阳性，而Rotaclone和TestPack分别检测出127个和129个阳性。ICS-RV的敏感性、特异性、阳性预测值和阴性预测值分别为94.0%、100%、100%和93.4%；Rotaclone分别为95.5%、100%、100%和95.0%；TestPack分别为97.0%、96.5%、97.0%和96.5%。ICS-RV灵敏且特异，操作和判读相对简单。
