Tricoli J V
Department of Radiation Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
Cancer Genet Cytogenet. 1999 May;111(1):1-6. doi: 10.1016/s0165-4608(98)00212-x.
A change in Y chromosome number is but one of the many cytogenetic abnormalities reported in human prostate tumors. However, reports in the literature have varied regarding the frequency of Y loss or gain, whether it is restricted to the cancerous tissue, and its relation to the biology of the disease. The most frequently used materials for analysis of Y enumeration have been metaphase spreads from short-term cell cultures of prostate tumor tissue and paraffin-embedded tissue sections. Analysis of Y chromosome number by using metaphase spreads on short-term cultures can be misleading owing to clonal cell selection during the establishment of these cultures. This may result in an incomplete representation of the loss/gain pattern in the tumor as a whole. Studies using paraffin-embedded tissue sections can be complicated by apparent chromosome loss due to nuclear truncation as a result of tumor sectioning. In an attempt to circumvent these problems, we have used touch preparations from human prostate tumors to search for Y chromosome loss. Fluorescence in situ hybridization analysis was conducted by using a whole chromosome Y paint, with an alpha-satellite chromosome 3 probe as a control, on tumor samples from 42 patients ages 40-75. The results demonstrated a gain of Y in a single prostate tumor sample, with no convincing evidence for loss of the entire Y chromosome in any of the other 41 samples examined. The results suggest that loss of the entire Y chromosome is an infrequent event in prostate cancer.
Y染色体数量的改变只是人类前列腺肿瘤中报告的众多细胞遗传学异常之一。然而，文献中的报告在Y染色体丢失或增加的频率、是否仅限于癌组织及其与疾病生物学的关系方面存在差异。用于Y染色体计数分析的最常用材料是前列腺肿瘤组织短期细胞培养物的中期染色体铺展和石蜡包埋组织切片。由于在这些培养物建立过程中的克隆细胞选择，使用短期培养物的中期染色体铺展来分析Y染色体数量可能会产生误导。这可能导致整个肿瘤中丢失/增加模式的不完全呈现。使用石蜡包埋组织切片的研究可能会因肿瘤切片导致的核截断引起的明显染色体丢失而变得复杂。为了规避这些问题，我们使用了人类前列腺肿瘤的触片来寻找Y染色体丢失。对42名年龄在40至75岁患者的肿瘤样本，使用全染色体Y探针进行荧光原位杂交分析，并以α卫星染色体3探针作为对照。结果显示在单个前列腺肿瘤样本中Y染色体增加，在所检测的其他41个样本中均未发现令人信服的整个Y染色体丢失的证据。结果表明，整个Y染色体丢失在前列腺癌中是罕见事件。
