Zaheer A, Weiss J L, Goyal P, Lim R
Department of Neurology, University of Iowa College of Medicine and Veterans Affairs Medical Center, Iowa City 52242, USA.
Neurosci Lett. 1999 Apr 23;265(3):203-6. doi: 10.1016/s0304-3940(99)00253-0.
Glia maturation factor (GMF) is a 17-kDa protein unique to the nervous system. Although GMF was initially characterized as a growth/differentiation factor, the absence of a leader sequence and its intracellular localization in normal brain suggest an intracellularfunction as well. In this paper we transfected the C6 glioma cells with GMF cDNA by infecting the cells with a GMF/adenovirus construct. The transfected cells overexpressed GMF but did not secret the protein into the culture medium. However, the transfected cells showed an increased expression of the neurotrophic factors including nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). The increase in neurotrophic activity of the C6 cell conditioned medium was demonstrable by its ability to promote neurite outgrowth in PC12 cells.
胶质细胞成熟因子（GMF）是一种神经系统特有的17 kDa蛋白。尽管GMF最初被表征为一种生长/分化因子，但它缺乏前导序列且在正常大脑中的细胞内定位表明它也具有细胞内功能。在本文中，我们通过用GMF/腺病毒构建体感染细胞，将GMF cDNA转染到C6胶质瘤细胞中。转染后的细胞过度表达GMF，但不将该蛋白分泌到培养基中。然而，转染后的细胞显示出包括神经生长因子（NGF）和脑源性神经营养因子（BDNF）在内的神经营养因子表达增加。C6细胞条件培养基的神经营养活性增加可通过其促进PC12细胞神经突生长的能力来证明。
