Barber P H
Department of Integrative Biology, University of California, Berkeley 94720, USA.
Mol Ecol. 1999 Apr;8(4):547-62. doi: 10.1046/j.1365-294x.1999.00593.x.
Patterns of phylogeography and gene flow were examined in the canyon treefrog, Hyla arenicolor. A total of 973 bp of mitochondrial cytochrome b sequence data were obtained for 65 individuals from 53 populations, yielding 50 unique haplotypes. Interpopulation sequence variation ranged from 0 to 13.7%. Phylogenetic analysis revealed three deeply divergent mtDNA lineages. These three Clades were mapped onto geography and found to represent completely concordant, nonoverlapping, geographical regions. Levels of sequence divergence between the three Clades were equal to or greater than published levels of divergence found in other vertebrate species and genera. Furthermore, one Clade of H. arenicolor was found to be more closely related to the outgroup H. eximia than to other H. arenicolor, suggesting that the taxonomy of this species may require revision.
对峡谷雨蛙（Hyla arenicolor）的系统地理学模式和基因流进行了研究。从53个种群的65个个体中获得了总共973bp的线粒体细胞色素b序列数据，产生了50个独特的单倍型。种群间的序列变异范围为0至13.7%。系统发育分析揭示了三个深度分化的线粒体DNA谱系。这三个分支被映射到地理区域上，发现它们代表了完全一致、不重叠的地理区域。这三个分支之间的序列分歧水平等于或大于在其他脊椎动物物种和属中发现的已发表的分歧水平。此外，发现一个峡谷雨蛙分支与外群艾氏雨蛙（H. eximia）的关系比与其他峡谷雨蛙的关系更密切，这表明该物种的分类可能需要修订。
