Wong R W, Rabie A B
University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China.
Eur J Orthod. 1999 Apr;21(2):119-26. doi: 10.1093/ejo/21.2.119.
The aim of the study was to assess quantitatively the amount of new bone formed in the early stages of healing of intramembranous and endochondral autogenous bone grafts so as to gain further insight into their integration with host bone. Eighteen critical size defects were created in the parietal bone of nine New Zealand White rabbits. In the experimental group (five rabbits), each rabbit was grafted with intramembranous bone in one defect and with endochondral bone in the other. In the control group (four rabbits), one defect was left empty (passive control) and the other was grafted with rabbit skin collagen (active control). After 14 days, the rabbits were killed and the defects were prepared for histological analysis. Serial sections were made across the whole defect. Each defect was divided into five regions spaced 1500 microns apart. Two sections were randomly drawn from each region. Quantitative analysis was performed on 100 sections using an image analyser computer software system to assess the amount of new bone formed in each defect. No bone was detected across the defect in either the active or passive controls. One-hundred-and-sixty-six per cent more new bone was formed in defects grafted with intramembranous bone than those grafted with endochondral bone. This represented an extremely significant difference (P < 0.0001, unpaired t-test) between the two groups. The results show that intramembranous autogenous bone produced more bone than the endochondral bone when grafted in the skull. Clinically, it is recommended that intramembranous bone is used to replace lost membranous bone in the oral cavity, as well as in skull defects, whenever possible.
本研究的目的是定量评估膜内和软骨内自体骨移植愈合早期形成的新骨量，以便进一步了解它们与宿主骨的整合情况。在9只新西兰白兔的顶骨上制造了18个临界尺寸缺损。在实验组（5只兔子）中，每只兔子的一个缺损处植入膜内骨，另一个缺损处植入软骨内骨。在对照组（4只兔子）中，一个缺损处留空（被动对照），另一个缺损处植入兔皮胶原蛋白（主动对照）。14天后，处死兔子并对缺损处进行组织学分析准备。制作贯穿整个缺损的连续切片。每个缺损被分成5个区域，间隔1500微米。从每个区域随机抽取2张切片。使用图像分析仪计算机软件系统对100张切片进行定量分析，以评估每个缺损处形成的新骨量。在主动或被动对照的缺损处均未检测到骨组织。与植入软骨内骨的缺损相比，植入膜内骨的缺损处形成的新骨多166%。这两组之间存在极其显著的差异（P < 0.0001，非配对t检验）。结果表明，当植入颅骨时，膜内自体骨比软骨内骨产生更多的骨组织。临床上，建议尽可能使用膜内骨来替代口腔以及颅骨缺损中缺失的膜性骨。
