Chay S H, Rabie A B, Itthagarun A
Faculty of Dentistry, National University of Singapore.
Aust Orthod J. 2000 Jul;16(2):88-97.
Alveolar bone defects are conditions that impede the progress of orthodontic treatment. This study compared the mechanics of the healing of autogenous intramembranous (IM) bone grafts and grafts comprising a mixture of IM and demineralised bone matrix of autogenous intramembranous origin (DBMIM), in an attempt to determine the reliability of each material. Thirty-two New Zealand white rabbits had a single defect created in their skull. Sixteen were grafted with IM bone alone (Group I: autogenous IM), and the other 16 had a combined graft of composite IM sandwiched between two layers of DBMIM (Group II: composite IM-DBMIM). A third group (Group III) of eight rabbits each had two defects created in their skull; one defect was left empty (A: passive control) and the other filled with rabbit-skin collagen (B: active control). In Groups I and II, inflammatory cells were found to be present on Days 1 and 2 of tissue retrieval. The appearance of the mesenchymal cells and preosteoblasts, osteoblasts and osteocytes was earlier (Day 3) in Group II than in Group I (Day 5). In both groups, preosteoblasts, osteoblasts and osteocytes were observed with no cartilage at the intermediate stage. In conclusion, autogenous IM bone grafts and IM bone grafts in the presence of DBMIM healed through an osteogenic ossification route.
牙槽骨缺损是阻碍正畸治疗进展的病症。本研究比较了自体膜内（IM）骨移植和包含自体膜内来源的IM与脱矿骨基质（DBMIM）混合物的移植材料的愈合机制，试图确定每种材料的可靠性。32只新西兰白兔在其颅骨上制造了单个缺损。16只仅用IM骨移植（第一组：自体IM），另外16只在两层DBMIM之间植入复合IM的联合移植材料（第二组：复合IM-DBMIM）。第三组（第三组）的8只兔子每只在其颅骨上制造两个缺损；一个缺损不进行处理（A：被动对照），另一个用兔皮胶原蛋白填充（B：主动对照）。在第一组和第二组中，在组织取材的第1天和第2天发现有炎症细胞。间充质细胞、前成骨细胞、成骨细胞和骨细胞的出现，第二组（第3天）比第一组（第5天）更早。在两组中，中间阶段均观察到前成骨细胞、成骨细胞和骨细胞，无软骨形成。总之，自体IM骨移植和存在DBMIM的IM骨移植通过成骨骨化途径愈合。
