Tekerlekov P, Dilovski M, Enchev S, Peneva I
Vet Med Nauki. 1978;15(3):19-25.
Coggins' immune diffusion test was modified, and was applied as a screening one in the study of the epizootic status. The positive reactions were characterized by the production of a precipitation line between the antigen and the respective serum that was tested. The appearance of such a line was associated with that formed with the use of the positive control serum, pointing to a reaction of identity. With the weakly positive reactions the ends of the precipitin lines, formed with the use of the positive control serum, were found to deviate slightly toward the site where the antigen had been placed. The specificity of the test was confirmed through biological experiments and clinical, morphological, and hematological investigations.
科金斯免疫扩散试验经过改良，在动物疫病流行状况研究中用作筛查试验。阳性反应的特征是在抗原与所检测的相应血清之间产生沉淀线。出现这样一条线与使用阳性对照血清形成的线相关，表明是同一性反应。对于弱阳性反应，发现使用阳性对照血清形成的沉淀线末端略微偏向放置抗原的部位。该试验的特异性通过生物学实验以及临床、形态学和血液学研究得以证实。
