Cao Long-Long, Xie Jian-Wei, Lin Yao, Zheng Chao-Hui, Li Ping, Wang Jia-Bin, Lin Jian-Xian, Lu Jun, Chen Qi-Yue, Huang Chang-Ming
Department of Gastric Surgery, Fujian Medical University Union Hospital Fuzhou 350001, Fujian Province, People's Republic of China.
College of Life Sciences, Fujian Normal University Fuzhou 350108, Fujian Province, People's Republic of China.
Int J Clin Exp Pathol. 2014 Aug 15;7(9):5582-94. eCollection 2014.
miR-183, a member of an evolutionarily conserved miRNA cluster (miR-96, miR-182, and miR-183), has been demonstrated to act as both a tumor suppressor and oncogene in various type of human cancer. However, the biological role of miR-183 in gastric cancer (GC) still remains unclear. In the present study, miR-183 expression was significantly decreased in gastric cancer tissues compared with its' adjacent normal tissues, and down-regulation of miR-183 was significantly associated with lymph node metastasis and pathological TNM stage. Furthermore, Erzin, which was reported to be up-regulated in gastric cancer, was identified as an efficient target of miR-183. Overexpression of miR-183 markedly suppressed cells invasion by downregulation of Ezrin expression. However, miR-183 expression didn't affect cells proliferation and cell cycle distribution of GC. In conclusion, our study demonstrated that miR-183 acts as a tumor suppressor in GC, partially at least via regulation of Ezrin. Therefore, miR-183 may be a potential target for the treatment of gastric cancer.
miR-183是进化上保守的miRNA簇（miR-96、miR-182和miR-183）的成员之一，已被证明在多种类型的人类癌症中既作为肿瘤抑制因子又作为癌基因发挥作用。然而，miR-183在胃癌（GC）中的生物学作用仍不清楚。在本研究中，与相邻正常组织相比，miR-183在胃癌组织中的表达显著降低，且miR-183的下调与淋巴结转移和病理TNM分期显著相关。此外，据报道在胃癌中上调的埃兹蛋白（Erzin）被确定为miR-183的有效靶标。miR-183的过表达通过下调埃兹蛋白的表达显著抑制细胞侵袭。然而，miR-183的表达不影响GC细胞的增殖和细胞周期分布。总之，我们的研究表明miR-183在GC中作为肿瘤抑制因子发挥作用，至少部分是通过调控埃兹蛋白实现的。因此，miR-183可能是胃癌治疗的潜在靶点。
