Gilmore-Hebert M A, Heywood S M
Biochim Biophys Acta. 1976 Nov 12;454(1):55-66. doi: 10.1016/0005-2787(76)90354-3.
Tubulin messenger RNA has been partially purified from embryonic chick brain. This messenger has been shown to be polyadenylated and capable of directing tubulin synthesis in an heterologous cell-free protein synthesizing system. Phosphocellulose fractions of IF-3 derived from embryonic leg muscle or brain were tested for their effect on tubulin and myosin synthesis in vitro. Phosphocellulose fraction four from either tissue source stimulates tubulin synthesis three fold. Myosin synthesis is enhanced significantly only by the muscle subfraction. This result suggests the existence of specific factors in muscle for the translation of the myosin messenger.
微管蛋白信使核糖核酸已从鸡胚脑中部分纯化出来。这种信使核糖核酸已被证明是聚腺苷酸化的，并且能够在异源无细胞蛋白质合成系统中指导微管蛋白的合成。对来自胚胎腿部肌肉或大脑的IF-3的磷酸纤维素级分进行了体外对微管蛋白和肌球蛋白合成影响的测试。来自任一组织来源的磷酸纤维素级分四可刺激微管蛋白合成三倍。只有肌肉亚级分能显著增强肌球蛋白的合成。这一结果表明肌肉中存在特定因子用于肌球蛋白信使核糖核酸的翻译。
