盘基网柄菌的17S、25S和5S核糖体DNA位于一个38,000碱基对的重复单元内。
Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit.
作者信息
Maizels N
出版信息
Cell. 1976 Nov;9(3):431-8. doi: 10.1016/0092-8674(76)90088-x.
Mapping with the restriction enzymes Sal 1 and R1 has generated a picture of the organization of Dictyostelium ribosomal DNA. The DNA which codes for 17S and 25S ribosomal RNAs is located within a stretch of repetitive DNA at least 38,000 base pairs long. This repeated unit includes 5S DNA, linked to 25S DNA. Two techniques were especially useful in the mapping: "cloning" 14S + 25S DNA on the plasmid pMB9 to amplify individual R1 fragments, and digesting DNA with R1 in the presence of the antibiotic distamycin A to produce specific partial digestion products.
用限制性内切酶Sal 1和R1进行图谱分析，得到了盘基网柄菌核糖体DNA的组织图谱。编码17S和25S核糖体RNA的DNA位于一段至少38000个碱基对长的重复DNA区域内。这个重复单元包括与25S DNA相连的5S DNA。两种技术在图谱分析中特别有用：将14S + 25S DNA“克隆”到质粒pMB9上以扩增单个R1片段，以及在抗生素偏端霉素A存在的情况下用R1消化DNA以产生特定的部分消化产物。
Zotero 插件