Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
Plant Mol Biol. 1983 Jan;2(1):15-8. doi: 10.1007/BF00187571.
RNA was extracted from plants infected with avocado sunblotch viroid (ASBV) and was analyzed by electrophoresis in polyacrylamide gel. The ASBV related fraction was eluted from the gel, labelled with [(32)P] using polynucleotide kinase and used as a probe for hybridization with a purified ASBV-RNA preparation dot spotted on nitrocellulose paper. Positive self-hybridization indicated a high degree of internal complementarity. Dot spots of whole cell RNA and of leaf sap from ASBV infected plants were shown to hybridize with the labelled probe. This hybridization procedure proved to be 16-64 times more sensitive in diagnosing ASBV when compared with polyacrylamide gel analysis.
从感染鳄梨日斑类病毒 (ASBV)的植物中提取 RNA，并用聚丙烯酰胺凝胶进行电泳分析。从凝胶中洗脱与 ASBV 相关的部分，用多核苷酸激酶标记 [(32)P]，并用作与点样在硝酸纤维素纸上的纯化 ASBV-RNA 制剂杂交的探针。阳性自杂交表明具有高度的内部互补性。来自感染 ASBV 的植物的整个细胞 RNA 和叶汁的斑点与标记探针杂交。与聚丙烯酰胺凝胶分析相比，该杂交程序在诊断 ASBV 时的灵敏度提高了 16-64 倍。
