Blumenthal R, Morris S J
Laboratory of Experimental and Computational Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Mol Membr Biol. 1999 Jan-Mar;16(1):43-7. doi: 10.1080/096876899294742.
To define the stages in influenza haemagglutinin (HA)-mediated fusion the kinetics of fusion between cell pairs consisting of single influenza HA-expressing cells and single erythrocytes (RBC) which had been labelled with both a fluorescent lipid (Dil) in the membrane and a fluorescent solute (calcein) in the aqueous space have been monitored. It is shown that release of solute from the target cell occurs, following the formation of the hemi-fusion diaphragm. These results are discussed in terms of a model in which fusion peptide insertion into the target membrane induces lipid stalks, which results in the formation of a hemifusion diaphragm and a fusion pore. Bilayer expansion due to overproduction of these stalks can give rise to collateral damage of target membranes.
为了定义流感血凝素（HA）介导的融合阶段，对由单个表达流感HA的细胞和单个红细胞（RBC）组成的细胞对之间的融合动力学进行了监测，其中红细胞的膜用荧光脂质（Dil）标记，水相空间用荧光溶质（钙黄绿素）标记。结果表明，在半融合隔膜形成后，靶细胞中的溶质会释放出来。根据一个模型对这些结果进行了讨论，在该模型中，融合肽插入靶膜会诱导脂质柄的形成，从而导致半融合隔膜和融合孔的形成。这些柄的过度产生导致的双层膨胀可能会对靶膜造成附带损伤。
