人体对马来丝虫病中马来布鲁线虫抗原的抗体反应。

Human antibody responses to Brugia malayi antigens in brugian filariasis.

作者信息

Zhang S, Li B, Weil G J

机构信息

Department of Internal Medicine, Washington University School of Medicine and Barnes-Jewish Hospital, St. Louis, MO 63110, USA.

出版信息

Int J Parasitol. 1999 Mar;29(3):429-36. doi: 10.1016/s0020-7519(98)00225-2.

DOI:10.1016/s0020-7519(98)00225-2

PMID:10333326

Abstract

Human antibody responses to Brugia malayi antigens were studied with sera from a Brugia endemic area in South India. Patients with clinical filariasis had significantly higher IgE and lower IgG4 levels to adult worm antigens than people with asymptomatic microfilaraemia. Intermediate antibody levels were observed in endemic normals. A majority of sera from each clinical group contained IgG antibodies to surface antigens of infective larvae (L3) by IFAT. IgG immunoblot studies did not reveal group differences in L3 antigen recognition. IgE antibodies bound to a subset of antigens bound by IgG. IgE antibodies in sera from clinical filariasis patients preferentially bound to L3 antigens at 200, 97, 68 and 58 kDa compared with sera from microfilaria carriers. These results are consistent with prior studies of antibody responses in filariasis and add new information on the targets of IgG and IgE antibodies to L3 antigens in brugian filariasis.

摘要

利用来自印度南部布鲁氏丝虫病流行地区的血清，研究了人体对马来布鲁线虫抗原的抗体反应。与无症状微丝蚴血症患者相比，临床丝虫病患者对成虫抗原的IgE水平显著更高，而IgG4水平更低。在地方性流行的正常人中观察到中等水平的抗体。通过间接荧光抗体试验（IFAT），每个临床组的大多数血清都含有针对感染性幼虫（L3）表面抗原的IgG抗体。IgG免疫印迹研究未揭示L3抗原识别方面的组间差异。IgE抗体与IgG结合的一部分抗原结合。与微丝蚴携带者的血清相比，临床丝虫病患者血清中的IgE抗体优先结合200、97、68和58 kDa的L3抗原。这些结果与之前关于丝虫病抗体反应的研究一致，并为布鲁氏丝虫病中IgG和IgE抗体针对L3抗原的靶点提供了新信息。