Tripathi Praveen Kumar, Mahajan Ramesh Chander, Malla Nancy, Kumar Naveen, Bhattacharya Shailja Misra, Shinoy Ranganatha Krishna, Mewara Abhishek, Sehgal Rakesh
Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Division of Parasitology, CDRI, Lucknow, Uttar Pradesh, India.
Trop Parasitol. 2017 Jan-Jun;7(1):29-36. doi: 10.4103/2229-5070.202298.
T-cell hypo-responsiveness in microfilaria (Mf) carriers against the microfilarial stage antigen of has been described, but no study has been carried out to assess antibody dynamics against stage-specific antigens.
The work was carried out with the aim to assess stage-specific antibody responses against L3 and microfilarial stage antigens in brugian filariasis in an endemic area.
Patients with different clinical spectra of brugian filariasis were recruited to evaluate antibody responses to brugian antigens.
Serum samples were collected from patients with different clinical spectra and antibody response was evaluated for total immunoglobulin G (IgG), IgG isotypes (IgG1, IgG2, IgG3, IgG4) and immunoglobulin E (IgE) response to L3 and microfilarial stage by enzyme-linked immunosorbent assay.
Paired t-test and one-way analysis of variance were carried out to analyze the data.
L3 and microfilarial stage antigens showed almost similar antibody responses in adenolymphangitis (ADL) and chronic pathology (CP) patients, however, diminished antibody response was observed with Mf stage antigen, especially with microfilaraemia. ADL patients had minimum antibody levels of all isotypes except IgG2 on day 0 which showed an increase subsequently, indicating suppression of antibody response during filarial fever. CP patients showed increase in IgE and decrease in IgG4 antibodies on day 365 indicating that these differences may be due to recent conversion into CP.
A prominent hyporesponsiveness in microfilaraemic individuals against microfilarial stage, but not against the L3 stage of the same parasite was observed, concluding stage-specificity in humoral immune response in brugian filariasis.
已有研究描述了微丝蚴（Mf）携带者针对班氏丝虫微丝蚴期抗原的T细胞低反应性，但尚未开展评估针对阶段特异性抗原的抗体动态变化的研究。
开展此项研究旨在评估流行区布鲁氏丝虫病患者针对L3和微丝蚴期抗原的阶段特异性抗体反应。
招募具有不同临床谱的布鲁氏丝虫病患者，以评估其对布鲁氏丝虫抗原的抗体反应。
采集具有不同临床谱患者的血清样本，通过酶联免疫吸附测定法评估针对L3和微丝蚴期的总免疫球蛋白G（IgG）、IgG亚型（IgG1、IgG2、IgG3、IgG4）和免疫球蛋白E（IgE）的抗体反应。
采用配对t检验和单因素方差分析进行数据分析。
L3和微丝蚴期抗原在腺淋巴管炎（ADL）和慢性病变（CP）患者中显示出几乎相似的抗体反应，然而，观察到Mf期抗原的抗体反应减弱，尤其是在微丝蚴血症患者中。ADL患者在第0天除IgG2外所有亚型的抗体水平最低，随后IgG2水平升高，表明在丝虫热期间抗体反应受到抑制。CP患者在第365天显示IgE升高和IgG4抗体降低，表明这些差异可能是由于近期转变为CP所致。
观察到微丝蚴血症个体对微丝蚴期存在显著的低反应性，但对同一寄生虫的L3期无低反应性，这表明布鲁氏丝虫病体液免疫反应具有阶段特异性。
