Kurniawan L, Basundari E, Fuhrman J A, Turner H, Purtoma H, Piessens W F
Immunology Division, National Institute of Health, Research and Development of the Ministry of Health, Jakarta, Indonesia.
Parasite Immunol. 1990 Mar;12(2):213-28. doi: 10.1111/j.1365-3024.1990.tb00949.x.
Studies in animal models indicate that antibodies to surface antigens of microfilariae participate in the control of parasitaemia resulting from infections with lymphatic filarial nematodes. In an attempt to identify parasite antigens that elicit such 'protective' host responses, we compared the antigen recognition patterns of persons who remained amicrofilaraemic after 3-6 years of exposure to Brugia malayi with those of individuals who developed patent filariasis during the same period. IgG antibodies in sera from immigrants identified between 0 and 25 microfilarial antigens on Western blots. The highest degree of reactivity was observed with antigens in the 65-75 kD and 20-30 kD ranges, and with a group of high mol. wt antigens (greater than 180 kD). Sera from amicrofilaraemic donors preferentially reacted with 70/75 kD microfilarial antigens. A proportion of such sera inhibited the binding of monoclonal antibody MF1 to its target epitope; eight of nine inhibitory sera were from patients with active infections, evidenced by the presence of microfilariae or filarial antigens in the donors' blood, but who were amicrofilaraemic. These results indicate that some amicrofilaraemic residents of areas where brugian filariasis is endemic develop immune reactions to a microfilarial stage-specific antigen that was previously identified as a potentially 'protective' parasite antigen in animal models of lymphatic filariasis.
对动物模型的研究表明，抗微丝蚴表面抗原的抗体参与控制由淋巴丝虫线虫感染引起的寄生虫血症。为了鉴定引发这种“保护性”宿主反应的寄生虫抗原，我们比较了暴露于马来布鲁线虫3至6年后仍无微丝蚴血症的人群与同期发生显性丝虫病的个体的抗原识别模式。在免疫印迹中，来自移民血清中的IgG抗体识别出0至25种微丝蚴抗原。在65 - 75 kD和20 - 30 kD范围内的抗原以及一组高分子量抗原（大于180 kD）观察到最高程度的反应性。无微丝蚴血症供体的血清优先与70/75 kD微丝蚴抗原发生反应。一部分此类血清抑制单克隆抗体MF1与其靶表位的结合；九份抑制性血清中有八份来自有活动性感染的患者，其证据是供体血液中存在微丝蚴或丝虫抗原，但他们无微丝蚴血症。这些结果表明，在马来丝虫病流行地区，一些无微丝蚴血症的居民对一种微丝蚴阶段特异性抗原产生免疫反应，该抗原先前在淋巴丝虫病动物模型中被鉴定为一种潜在的“保护性”寄生虫抗原。
