Fan G K, Fujieda S, Sunaga H, Tsuzuki H, Ito N, Saito H
Department of Otorhinolaryngology, Fukui Medical University, Japan.
Laryngoscope. 1999 May;109(5):815-20. doi: 10.1097/00005537-199905000-00025.
A cyclin-dependent kinase inhibitor, p27kip1, is recognized as a negative regulator of the cell cycle. To clarify whether immunohistochemical detection of p27 might provide prognostic information, we investigated the expression of p27 in laryngeal squamous cell carcinoma (SCC).
A retrospective study of patients was performed in 109 cases of laryngeal SCC. In addition, we investigated the expression of p53 and granulocyte colony-stimulating factor receptor (GCSF-R) to examine the prognostic significance of them in the same samples.
Immunohistochemical staining by specific monoclonal antibodies was performed using the avidin-biotin-peroxidase complex technique.
Advanced tumor size and clinical stage and the occurrence of lymph node metastasis were associated with the absence of p27 expression, but not correlated with p53 expression and GCSF-R expression. The overall 5-year survival rate in the p27-positive group was significantly higher than that in the p27-negative group. In the Cox proportional hazard model, p27 was demonstrated to be the most powerful prognostic factor among gender, tumor size, lymph node metastasis, stage of disease, and p53 and GCSF-R expression.
We concluded that assessment of p27 expression is useful as a prognostic factor for laryngeal SCC and of value in selecting patients with laryngeal SCC for aggressive therapy.
细胞周期蛋白依赖性激酶抑制剂p27kip1被认为是细胞周期的负调节因子。为了阐明p27的免疫组化检测是否能提供预后信息，我们研究了p27在喉鳞状细胞癌（SCC）中的表达。
对109例喉SCC患者进行回顾性研究。此外，我们研究了p53和粒细胞集落刺激因子受体（GCSF-R）的表达，以检验它们在相同样本中的预后意义。
采用抗生物素蛋白-生物素-过氧化物酶复合物技术，用特异性单克隆抗体进行免疫组化染色。
肿瘤体积大、临床分期晚以及发生淋巴结转移与p27表达缺失相关，但与p53表达和GCSF-R表达无关。p27阳性组的总体5年生存率显著高于p27阴性组。在Cox比例风险模型中，p27被证明是性别、肿瘤大小、淋巴结转移、疾病分期以及p53和GCSF-R表达中最有力的预后因素。
我们得出结论，评估p27表达作为喉SCC的预后因素是有用的，并且对于选择喉SCC患者进行积极治疗具有价值。
