由结合 GTP 的 Ran 在非洲爪蟾卵提取物中诱导产生的微管星状体的自组织。

Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran.

作者信息

Ohba T, Nakamura M, Nishitani H, Nishimoto T

机构信息

Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Maidashi Higashi-ku, Fukuoka 812-8582, Japan.

出版信息

Science. 1999 May 21;284(5418):1356-8. doi: 10.1126/science.284.5418.1356.

DOI:10.1126/science.284.5418.1356

PMID:10334990

Abstract

The nucleotide exchange activity of RCC1, the only known nucleotide exchange factor for Ran, a Ras-like small guanosine triphosphatase, was required for microtubule aster formation with or without demembranated sperm in Xenopus egg extracts arrested in meiosis II. Consistently, in the RCC1-depleted egg extracts, Ran guanosine triphosphate (RanGTP), but not Ran guanosine diphosphate (RanGDP), induced self-organization of microtubule asters, and the process required the activity of dynein. Thus, Ran was shown to regulate formation of the microtubule network.

摘要

RCC1是与Ras类似的小GTP酶Ran唯一已知的核苷酸交换因子，在减数分裂II期停滞的非洲爪蟾卵提取物中，无论有无去膜精子，微管星状体的形成都需要RCC1的核苷酸交换活性。同样，在耗尽RCC1的卵提取物中，三磷酸鸟苷Ran（RanGTP）而非二磷酸鸟苷Ran（RanGDP）可诱导微管星状体的自组装，且该过程需要动力蛋白的活性。因此，Ran被证明可调节微管网络的形成。