Muller E H, Richards E J, Norbeck J, Byrne K L, Karlsson K A, Pretorius G H, Meacock P A, Blomberg A, Hohmann S
Department of Cell and Molecular Biology/Microbiology, Göteborg University, Sweden.
FEBS Lett. 1999 Apr 23;449(2-3):245-50. doi: 10.1016/s0014-5793(99)00449-4.
The Saccharomyces cerevisiae gene PDC5 encodes the minor isoform of pyruvate decarboxylase (Pdc). In this work we show that expression of PDC5 but not that of PDC1, which encodes the major isoform, is repressed by thiamine. Hence, under thiamine limitation both PDC1 and PDC5 are expressed. PDC5 also becomes strongly expressed in a pdc1delta mutant. Two-dimensional gel electrophoresis of whole protein extracts shows that thiamine limitation stimulates the production of THI gene products and of Pdc5p. Deletion of PDC1 only stimulates production of Pdc5p. We conclude that the stimulation of PDC5 expression in a pdc1delta mutant is not due to a response to thiamine limitation.
酿酒酵母基因PDC5编码丙酮酸脱羧酶（Pdc）的次要同工型。在本研究中，我们发现硫胺素可抑制PDC5的表达，但不抑制编码主要同工型的PDC1的表达。因此，在硫胺素限制条件下，PDC1和PDC5均会表达。PDC5在pdc1δ突变体中也会强烈表达。全蛋白提取物的二维凝胶电泳显示，硫胺素限制会刺激THI基因产物和Pdc5p的产生。仅缺失PDC1会刺激Pdc5p的产生。我们得出结论，pdc1δ突变体中PDC5表达的刺激并非源于对硫胺素限制的反应。
