Gouet P, Diprose J M, Grimes J M, Malby R, Burroughs J N, Zientara S, Stuart D I, Mertens P P
The Laboratory of Molecular Biophysics, University of Oxford, United Kingdom.
Cell. 1999 May 14;97(4):481-90. doi: 10.1016/s0092-8674(00)80758-8.
The concentration of double-stranded RNA within the bluetongue virus core renders the genome segments liquid crystalline. Powder diffraction rings confirm this local ordering with a 30 A separation between strands. Determination of the structure of the bluetongue virus core serotype 10 and comparison with that of serotype 1 reveals most of the genomic double-stranded RNA, packaged as well-ordered layers surrounding putative transcription complexes at the apices of the particle. The outer layer of RNA is sufficiently well ordered by interaction with the capsid that a model can be built and extended to the less-ordered inner layers, providing a structural framework for understanding the mechanism of this complex transcriptional machine. We show that the genome segments maintain local order during transcription.
蓝舌病毒核心内双链RNA的浓度使基因组片段呈液晶态。粉末衍射环证实了这种局部有序性，链间间距为30埃。10型蓝舌病毒核心结构的测定以及与1型的比较表明，大部分基因组双链RNA包装成围绕颗粒顶端假定转录复合物的有序层。RNA的外层通过与衣壳的相互作用而充分有序，以至于可以构建一个模型并扩展到有序程度较低的内层，为理解这种复杂转录机器的机制提供了一个结构框架。我们表明，基因组片段在转录过程中保持局部有序。
Zotero 插件