Olczak M, Olczak T
Institute of Biochemistry and Molecular Biology, University of Wroclaw, Poland.
Clin Chim Acta. 1999 Apr;282(1-2):35-44. doi: 10.1016/s0009-8981(99)00010-8.
As pathophysiological functions claimed for Tamm-Horsfall protein (THP) are related to its sugar moiety, we examined influence of pregnancy and various diseases on high-mannose chains. Hydrazinolysis was used to liberate oligosaccharides from THP polypeptide backbone. After HPLC separation of fluorescently labelled glycans similar profiles of neutral oligosaccharides were observed in THP of healthy subjects, pregnant women, patients with Bartter's syndrome, patients with acute lymphoblastic leukemia and a patient with carbohydrate deficient glycoprotein syndrome. THP contains Man-5, Man-6 and Man-7 glycans, with the preponderant amount of Man-6 glycan (about 7% of total THP oligosaccharides). No statistically significant differences were found in THP high-mannose glycans profiles between control subjects and pregnant women or patients. It is likely that neither pregnancy nor the pathological conditions examined affect high-mannose chains. In our opinion hydrazinolysis as a method of oligosaccharides liberation, in contrast to enzymatic deglycosylation, is more appropriate for analysis of the sugar moiety of THP.
由于人们认为Tamm-Horsfall蛋白（THP）的病理生理功能与其糖部分有关，我们研究了妊娠和各种疾病对高甘露糖链的影响。采用肼解从THP多肽主链释放寡糖。在对荧光标记的聚糖进行高效液相色谱分离后，在健康受试者、孕妇、巴特综合征患者、急性淋巴细胞白血病患者和碳水化合物缺乏糖蛋白综合征患者的THP中观察到相似的中性寡糖谱。THP含有Man-5、Man-6和Man-7聚糖，其中Man-6聚糖含量最多（约占THP总寡糖的7%）。在对照组与孕妇或患者之间，THP高甘露糖聚糖谱未发现统计学上的显著差异。妊娠和所检查的病理状况可能均不影响高甘露糖链。我们认为，与酶促去糖基化相比，肼解作为一种释放寡糖的方法更适合于分析THP的糖部分。
