Olczak T, Olczak M, Kubicz A, Duława J, Kokot F
Institute of Biochemistry and Molecular Biology, University of Wrocław, Tamka 2, 50-137 Wrocław, Poland.
Int J Clin Lab Res. 1999;29(2):68-74. doi: 10.1007/s005990050066.
The sugar moiety of Tamm-Horsfall protein (THP) is altered by pathological conditions. The aim of this study was to investigate the composition of THP glycans in urinary diseases. THP was isolated from the urine of patients with urinary tract infection (group A), glomerulonephritis or interstitial nephritis (group B), and Bartter's syndrome (BS) (group C). Monosaccharides, N-glycan profile, THP reactivity with specific lectins and some other proteins were analyzed. THP of patients from groups A, B, and C showed lower amounts of N-acetylgalactosamine (P<0.05, P<0.005, and P<0.05, respectively) than controls; this was reflected in lower reactivity with Phaseolus vulgaris lectin (P<0.005, P<0.05, and P<0.005). Reduced amounts of N-acetylglucosamine were noticed in groups A (P<0. 05) and B (P<0.05). In group A lower amounts of galactose and alpha2, 6-linked sialic acid, as determined by reactivity with Datura stramonium lectin (P<0.005) and Sambucus nigra lectin (P<0.005), were observed. In patients with BS there was a shift from tetrasialylated glycans towards less-sialylated chains. We found also that THP of all patients binds more strongly to IgG(1) (P<0.005, for all patient groups). Our results indicate that the urinary diseases examined affect the THP sugar moiety and the binding of THP to IgG(1).
Tamm-Horsfall蛋白（THP）的糖部分会因病理状况而改变。本研究的目的是调查泌尿系统疾病中THP聚糖的组成。从患有尿路感染的患者（A组）、肾小球肾炎或间质性肾炎患者（B组）以及巴特综合征（BS）患者（C组）的尿液中分离出THP。分析了单糖、N-聚糖谱、THP与特定凝集素及其他一些蛋白质的反应性。A组、B组和C组患者的THP显示，与对照组相比，N-乙酰半乳糖胺含量较低（分别为P<0.05、P<0.005和P<0.05）；这反映在与菜豆凝集素的反应性较低（P<0.005、P<0.05和P<0.005）。在A组（P<0.05）和B组（P<0.05）中发现N-乙酰葡糖胺含量减少。在A组中，通过与曼陀罗凝集素（P<0.005）和黑接骨木凝集素（P<0.005）反应性测定，发现半乳糖和α2,6-连接唾液酸含量较低。在患有巴特综合征的患者中，存在从四唾液酸化聚糖向唾液酸化程度较低的链的转变。我们还发现，所有患者的THP与IgG(1)的结合更强（所有患者组P<0.005）。我们的结果表明，所检测的泌尿系统疾病会影响THP的糖部分以及THP与IgG(1)的结合。
